Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5161-5164.doi: 10.3969/j.issn.1673-8225.2011.28.008

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Xianlinggubao promotes vascular formation in osteoporotic fracture callus

Tian Fa-ming1, Zhang Liu1, 2, Luo Yang2, Song Ya-qi1, Jiao Ao1, Cheng Tan 2   

  1. 1Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan  063000, Hebei Province, China
    2Postgraduate School, Hebei Medical University, Shijiazhuang  050017, Hebei Province, China
  • Received:2011-01-15 Revised:2011-03-25 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhang Liu, Professor, Chief physician, Doctoral supervisor, Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China; Postgraduate School, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China zhliu130@sohu.com
  • About author:Tian Fa-ming☆, Doctor, Department of Orthopedics, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China tfm9911316@163.com

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Abstract:

BACKGROUND: Controversy still exists in impact of osteoporosis on early process of fracture healing, and the effect and related mechanism underlying Xianlinggubao (XLGB) on osteoporotic fracture healing need further study.
OBJECTIVE: To confirm the influence of osteoporosis on the healing of femoral shaft fracture and to investigate the effects of XLGB on osteoporotic fracture healing.
METHODS: Fifty female 12-week old Sprague-Dawley rats were randomly divided into 5 groups with 10 animals in each group: sham-surgery, fracture, ovariectomy (OVX), OVX + fracture and XLGB. OVX was performed in bilateral sides of rats. Transverse fracture model of femoral shaft was established at 4 weeks after OVX. Rats in XLGB treatment group were treated with 250 mg/kg XLGB daily for 3 weeks following the establishment of OVX and fracture. Rats in the OVX + fracture, fracture and XLGB treatment groups were harvested the femoral fracture samples for bone mineral density test by X-ray radiography. The pathological changes of callus tissues were detected with hematoxylin-eosin staining and blood vessels were counted. Immunohistochemical staining was applied to determine the expression of bone morphogenetic protein 2 in callus tissues.
RESULTS AND CONCLUSION: Bone mineral density test and computed X-ray radiography score showed a significantly reduction in the rats following OVX (P < 0.05). XLGB treatment could enhance the bone mineral density and X-ray radiography score in the OVX and fracture rats, with no significant difference (P > 0.05). XLGB treatment also increased the quantity of callus tissues compared with OVX + fracture and fracture groups (P < 0.05). No significant difference was observed of bone morphogenetic protein-2 expression among three groups. The ovariectomized rats show a delayed fracture healing process and XLGB could promote the early vascular formation of osteoporotic fracture healing.

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