Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5153-5156.doi: 10.3969/j.issn.1673-8225.2011.28.006

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Secretion of type Ⅱ collagen and hyaluronic acid in chondrocytes transfected by adenovirus-bone morphogenetic protein 7

Zhang Jie1, Liu Wei2, Zhu Xin-hui2, Zhou Yi1   

  1. 1Department of Immunology, Medical School of Nantong University, Nantong   226001, Jiangsu Province, China
    2Department of Joint Surgery, Second Affiliated Hospital of Nantong University, Nantong  226001, Jiangsu Province, China
  • Received:2011-04-22 Revised:2011-06-10 Online:2011-07-09 Published:2011-07-09
  • Contact: Liu Wei, Doctor, Chief physician, Department of Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China romly80@sina.com
  • About author:Zhang Jie☆, Doctor, Lecturer, Department of Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China zhangjie@ntu.edu.cn
  • Supported by:

    Society Development Science and Technology Plan Program of Nantong City, No. S2008042*; a grant from Open Project of Public Health Department of Jiangsu Province, No.KF200953*; a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institution*

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Abstract:

BACKGROUND: Chondrocytes are limited in tissue engineering due to their poor self-dividing capacity and defifferentiation.
OBJECTIVE: To investigate the expression of bone morphogenetic protein 7 (BMP-7) in chondrocytes transfected by adenovirus BMP-7 and the effects of transfection on chondrocyte secretion of typeⅡcollagen and hyaluronic acid.
METHODS: Adenoviral vector containing BMP-7 was prepared and then transfected into rabbit passage chondrocytes. BMP-7 mRNA and protein expressions in chondrocytes were detected. Changes in type Ⅱ collagen and hyaluronic acid in chondrocytes were determined.
RESULTS AND CONCLUSION: At 48 and 72 hours after transfection, RT-PCR and western blot analysis showed that BMP-7 mRNA and protein expressions in the chondrocytes were increased; RT-PCR and ELISA showed the type Ⅱ collagen and hyaluronic acid in the chondrocytes were also obviously increased. These findings suggest that BMP-7 can be successfully transfected into chondrocytes by adenovirus and promote the secretion of type Ⅱcollagen and hyaluronic acid.

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