Abstract:

BACKGROUND: Currently xenogeneic cancellous bone is more common as tissue engineering materials, but cortical bone is restricted due to degrading difficulty and low porosity. However, cortical bone is inferior to cancellous bone, such as bio-mechanics, how to develop the use of cortical bone is the focus of the research.
OBJECTIVE: To observe the expression of vascular endothelial growth factor (VEGF) after rabbit bone marrow mesenchymal stem cells (BMSCs) and cortical bone scaffold with partial cancellous bone were implanted into rabbits.
METHODS: Four healthy 1-month-old New Zealand white rabbits were used for stem cell extraction, and 60 3-month-old New Zealand rabbits were implanted with BMSCs induced osteogenic composite calf bone, simple bone xenograft group and autogenous iliac bone into ala of ilium. The expressions of VEGF were detected by RT-PCR at post-operative 4, 8, 12, 24 weeks.
RESULTS AND CONCLUSION: The VEGF expression in simple bone xenograft group was lower than that in BMSCs induced osteogenic composite calf bone group and autogenous iliac bone group at all time points (P < 0.05). At postoperative 4 weeks, the BMSCs induced osteogenic composite calf bone group had lower expression than autogenous iliac bone group (P < 0.05), but with no significant differences at postoperative 8, 12, 24 weeks (P < 0.05). Rabbit BMSCs combined with calf cortical bone scaffold with partial cancellous bone implanted into New Zealand rabbits have good angiogenesis capacity.