Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5185-5188.doi: 10.3969/j.issn.1673-8225.2011.28.014

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Expression of protein kinase C and heat shock protein 70 in the heart of rat models of long-term exercise preconditioning

Sun Xiao-juan, Hou Na   

  1. Department of Physical Education, Xianyang Normal University, Xianyang 712000, Shaanxi Province, China
  • Received:2011-03-03 Revised:2011-06-03 Online:2011-07-09 Published:2011-07-09
  • About author:Sun Xiao-jun☆, Doctor, Lecturer, Department of Physical Education, Xianyang Normal University, Xianyang 712000, Shaanxi Province, China sxj362@yahoo.com. cn
  • Supported by:

    Science Research Plan Program of Education Department of Shaanxi Province, No.09JK805*; Sport Science Research Program of Sport Bureau of Shaanxi Province in 2010, No. 10050*; Science Research Foundation for Xianyang Normal University, No. 09XSYK103*

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Abstract:

BACKGROUND: Several studies have demonstrated that protein kinase C (PKC) and heat shock protein 70 (HSP70) take part in the cardioprotection of exercise preconditioning (EP), but the relationship and the underlying mechanism remain poorly understood.
OBJECTIVE: To investigate the effects of long-term EP on PKC and HSP70 expression in the rat heart and the possible mechanism of cardioprotection.
METHODS: Sprague-Dawley rats were randomly divided into the control, exhausted exercise and EP groups. Rats in the control and exhausted exercise groups were fed normally for 3 weeks. The EP group rats underwent 3 weeks of intermittent swimming exercise in order to establish long-term EP animal model. After 3 weeks, the exhausted exercise group and exercise preconditioning group underwent exhausted exercise. PKC and HSP70 expression in rat heart was determined by western blot analysis and immunohistochemistry.
RESULTS AND CONCLUSION: After exhausted exercise, cardiac HSP70 expression was greater in the exhausted exercise group than in the control group (P < 0.05). After 3 weeks of EP and subsequent exhausted exercise, cardiac PKC and HSP70 expression was greater in the EP group than in the exhausted exercise group (P < 0.05). The results demonstrated that long-term EP could activate cardiac PKC expression and induce HSP70 synthesis, which could play a big role in cardioprotection of long-term EP.

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