Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5181-5184.doi: 10.3969/j.issn.1673-8225.2011.28.013

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Expression of interleukin-1 β and nuclear factor kappa B in rat models of acute spinal cord injury

Zong Shao-hui1, Wei Bo2, Zeng Gao-feng2, Zhao Yu-xi3, Xiong Chun-xiang3   

  1. 1Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2School of Public Health, 3School of Postgraduate, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-12-15 Revised:2011-02-22 Online:2011-07-09 Published:2011-07-09
  • About author:Zong Shao-hui,☆ Doctor, Associate professor, Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaohui3008@126. com
  • Supported by:

    Ph.D Programs Foundation of Guangxi Medical University, No. 308010*; Science Research Foundation of Ministry of Education of Guangxi Zhuang Autonomous Region, Guijiao 200710LX063*

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Abstract:

BACKGROUND: During the process of secondary spinal cord injury, interleukin-1β participates in stimulation of other cytokines and synthesis of injury medium.
OBJECTIVE: To investigate the effects of interleukin-1 receptor antagonist on the expression of interleukin-1β and nuclear factor-κB in rat models of acute spinal cord injury.
METHODS: Sprague-Dawley rat models of acute spinal cord injury were established by modified Allen method. After modeling, the injured spinal cord tissue was spread gelatin sponge containing interleukin-1 receptor antagonist or normal saline. At 1, 48, and 72 hours postoperatively, the injured spinal cord tissue samples were harvested for detection of interleukin-1β and nuclear factor κB by immunohistochemical method.
RESULTS AND CONCLSUION: After interleukin-1 receptor antagonist treatment, interleukin-1β and nuclear factor κB expression was greatly decreased. These findings suggest that interleukin-1 receptor antagonist can alleviate local inflammatory reaction by inhibiting interleukin-1β and nuclear factor κB expression, exerting protective effects on injured spinal cord segment in rat models of acute spinal cord injury.

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