Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4409-4414.doi: 10.3969/j.issn.1673-8225.2011.24.010

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Effects of berberine on expression of hepatic peroxisome proliferator-activated receptors and its target genes in type 2 diabetic Chinese hamsters

Liu Xu-han1, Li Guo-sheng 2, Huang Lan3, Zhu Hua3, Liu Ya-li3, Ma Chun-mei3   

  1. 1Department of Endocrinology, Dalian Municipal Central Hospital, Dalian 116003, Liaoning Province, China
    2Department of Pathology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
    3Department of Pathology, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing  100021, China
  • Received:2011-02-09 Revised:2011-05-10 Online:2011-06-11 Published:2011-06-11
  • Contact: Liu Xu-han☆, Doctor, Attending physician, Department of Endocrinology, Dalian Municipal Central Hospital, Dalian 116003, Liaoning Province, China xuhanliu281277@yahoo.com.cn Li Guo-sheng, Doctor, Attending physician, Department of Pathology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China guoshengli998@163.com Liu Xu-han and Li Guo-sheng were considered co-corresponding authors.
  • About author:Liu Xu-han☆, Doctor, Attending physician, Department of Endocrinology, Dalian Municipal Central Hospital, Dalian 116003, Liaoning Province, China xuhanliu281277@yahoo.com.cn

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Abstract:

BACKGROUND: Although Berberine has been reported to treat type 2 diabetes, the underlying mechanisms of berberine on insulin resistance of type 2 diabetes, especially hepatic insulin resistance, remains not fully understood.
OBJECTIVE: To study the effects of berberine on the expression of hepatic peroxisome proliferator-activated receptors (PPARs) and their target genes in type 2 diabetic Chinese hamsters.
METHODS: The insulin-resistant and type 2 diabetic Chinese hamster models were induced by high-fat diet without or with low-dose streptozotocin. After the induction of models, the hamsters were randomly divided into normal control (standard food), insulin-resistant (high-fat diet), diabetic (high-fat diet and streptozotocin) and berberine-treated diabetic (high-fat diet and streptozotocin and berberine) groups. All groups were treated for 9 weeks.
RESULTS AND CONCLUSION: Results of real-time quantitative PCR indicated that compared with normal control group, the expression of PPARα, PPARβ/δ, acyl-Coenzyme A oxidase (Acox), carnitine palmitoyltransferase 1 (Cpt1) and acetyl-Coenzyme A dehydrogenase, medium chain (Acadm) was decreased (P < 0.05) and the expression of sterol regulatory element binding factor 1 (SREBP1c), sterol regulatory element binding factor 2 (SREBP2), PPARγ, lipoprotein lipase (LPL), CD36/FA transporter (FAT/CD36) and adipocyte fatty acid-binding protein (ap2) was increased (P < 0.05) in the fatty liver of insulin-resistant and diabetic hamster groups. Berberine effectively improved insulin resistance, reversed the altered expression of PPARs and its target genes in diabetic hamsters. PPARs and its target genes involved in the therapeutic molecular mechanisms of berberine on fat-induced hepatic insulin resistance in type 2 diabetic hamsters.

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