Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3785-3788.doi: 10.3969/j.issn.1673-8225.2011.20.043

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Role of Fas, FasL and Caspase-3 in artesunate-induced apoptosis of human embryonic lung fibroblasts

Wang Chang-ming, Li Hong-xiu, Zhang Xiao-fei   

  1. Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-01-06 Revised:2011-04-09 Online:2011-05-14 Published:2011-05-14
  • Contact: Li Hong-xiu, Studying for master’s degree, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China 763460155@qq.com
  • About author:Wang Chang-ming☆, Doctor, Professor, Master’s supervisor, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China wcm@glmc.edu.cn Li Hong-xiu, Studying for master’s degree, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China 763460155@qq.com Wang Chang-ming and Li Hong-xiu contributed equally to this paper.
  • Supported by:

    Science Research and Technology Development Plan of Guangxi Province, No. 10124008-4*

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Abstract:

BACKGROUND: Artesunate can relieve pulmonary fibrosis, but its mechanisms are rarely reported.
OBJECTIVE: To explore the effect of artesunate on apoptosis of HFL-I cells and the role of Fas, FasL and Caspase-3 in the artesunate-mediated apoptosis of HFL-I cells.
METHODS: Cell counting kit-8 (CCK-8) assay was used to determine the effects of artesunate at 1, 10, 100 mg/L on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Fas, FasL and Caspase-3, were assessed by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: Artesunate had a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependent manner in vitro. The apoptosis rate of HFL-I cells was significantly increased in the artesunate-treated group compared with the control group (P < 0.05 or P  < 0.01). The mRNA levels of Fas, FasL and Caspase-3 were significantly higher in the artesunate-treated group than the control group (P < 0.05). The findings of this study demonstrate that artesunate can exert a marked anti-pulmonary fibrosis effect by up-regulating mRNA levels of Fas, FasL and Caspase-3, which can induce the growth inhibition and apoptosis in HFL-I cells.

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