Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3781-3784.doi: 10.3969/j.issn.1673-8225.2011.20.042

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Gene-gene interaction between transforming growth factor beta 3 and bone morphogenetic protein 4 in cleft lip with or without cleft palate

Ding Kai-hong, Ban Fu-zhi, Huang Cheng-le   

  1. Department of Chemical Examination, Baise People’s Hospital, Baise  533000, Guangxi Zhuang Autonomous Region, China
  • Received:2010-10-09 Revised:2010-12-11 Online:2011-05-14 Published:2011-05-14
  • About author:Ding Kai-long, Technician-in-charge, Department of Chemical Examination, Baise People’s Hospital, Baise 533000, Guangxi Zhuang Autonomous Region, China dingkaihong@yeah. net

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Abstract:

BACKGROUND: Our previous studies have demonstrated that cleft lip with or without cleft palate (CL/P) is associated with the polymorphism of bone morphogenetic protein 4 (BMP4) rather than transforming growth factor β3 (TGFβ3).
OBJECTIVE: To elucidate the interactions among T538C for BMP4, C641A and G15572- for TGFβ3 in CL/P. 
METHODS: The genotypes of BMP4 T538C, TGFβ3 C641A and TGFβ3 G15572- were detected in 200 CL/P patients and 200 controls using a polymerase chain reaction-restriction fragment length polymerase (PCR-RFLP) strategy. The interaction effects among these three studied loci were analyzed using the multifactor dimensionality reduction (MDR) method.
RESULTS AND CONCLUSION: Significant association of between genotype and allele distributions of the BMP4T538C and CL/P was revealed (P < 0.05), while no significant association of TGFβ3C641A and 15572G/- genotype or allele distribution was found (P > 0.05). In interaction analysis, significance level of all models was larger than 0.05, which shown no existence of interaction effect among BMP4T538C, TGFβ3 C641A and G15572-.

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