Abstract:

BACKGROUND: Previous research has demonstrated that nuclear factor (NF) κB mRNA and protein are both increased in eutopic and ectopic endometrium of patients with endometriosis. NF κB participates in the pathological process of cell adhesion, invasion and angiopoiesis in endometriosis.
OBJECTIVE: To investigate the effect of small interfering RNA (siRNA) targeting NF-ΚB on proliferation of human vascular endothelial cells (EAhy926 cells).
METHODS: EAhy926 cells were cultured with stimulation of vascular endothelial growth factor (VEGF, 10 μg/L), and then were transfected with NF-ΚB-siRNA. Meanwhile, negative control-siRNA, empty liposome and normal control groups were established. The proliferation inhibition and apoptosis of cells were detected by Heochst 33342 test, MMT assay and flow cytometry analysis. 
RESULTS AND CONCLUSION: Compared with the normal control group, the rates of proliferation inhibition and apoptosis of cells were increased obviously in the NF-ΚB p65-siRNA group (P < 0.05); there were no significant differences between empty liposome group, negative control siRNA group and normal control group, respectively (P > 0.05). Synthetic siRNA can effectively inhibit the proliferation and stimulates the apoptosis of human vascular endotheliar cells.