Abstract:

BACKGROUND: Previous experiments have showed that topoisomerase Ⅰ is highly expressed in hypertrophic scar fibroblasts. This experiment hypothesized that weather hydroxycamptothecin (HCPT), an inhibitor of topoisomerase Ⅰ, can inhibit human hypertrophic scar fibroblasts proliferation cultured in vitro?
OBJECTIVE: To investigate the effect of HCPT on the proliferation of fibroblasts isolated from human pathological scars.
METHODS: Pathological scar fibroblasts were cultured in vitro. MTT assay was used to determine the effect of HCPT on the proliferation of fibroblasts under 10 different doses (2-1 000 μg/L) at 24, 48 and 72 hours. 
RESULTS AND CONCLUSION: HCPT with low concentration (2-8 μg/L) exhibited inhibitory effects on fibroblasts proliferation, which reached a platform stage with the next concentration (8-125 μg/L), and the effect would enhanced at the high concentration (125-500 μg/L). It revealed that HCPT had inhibitory effects on fibroblasts proliferation in a concentration-dependent manner. The concentration of drug was positively correlated with cell inhibitory rate (r=0.87, P < 0.05). As the time prolonged, the inhibition was not increased. Half of the inhibition rates (IC50) were 233, 176 and 103 μg / L at 24, 48, and 72 hours. Accordingly, HCPT can inhibit proliferation of human pathological scar fibroblasts in vitro.