Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 6015-6019.doi: 10.3969/j.issn.1673-8225.2010.32.027

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Hematopoietic reconstruction in mice treated with lethal dose irradiation using Sca-1+ hematopoietic stem/progenitor cells transplantation 

Zhou Yue, Yang Bin, Yao Xin, Wang Ya-ping   

  1. Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing  400016, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Wang Ya-ping, Professor, Doctoral supervisor, Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China ypwangcq@yahoo.cn
  • About author:Zhou Yue☆, Studying for doctorate, Lecturer, Research Room of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China zhouyue_120@sina. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973818*; the Natural Science Foundation of Scientific Committee of Chongqing City, No.CSTC,2009BA5038*

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Abstract:

BACKGROUND: In recent years, hematopoietic stem cell transplantation has widely used in clinic. The effectiveness of HSC reconstruction of the hematopoiesis has became a hot topic in the field of HSCT.
OBJECTIVE: To study hematopoietic reconstruction for hematopoietic failure mice with the transplantation of Sca-1+ hematopoietic stem/progenitor cells (Sca-1+HSC/HPC).
METHODS: The female C57BL/6 recipient mice received a lethal dose irradiation from 60Coγ ray and were divided into two groups. The control group mice were injected with sterile phosphate buffered saline. The transplantation group mice were transplanted with Sca-1+HSC/HPC isolated by magnetic cell sorting from homologous series male mice. After transplantation, the survival time, the change of peripheral blood leucocyte, haematocrit and platelet, the weight of spleen, the number of colony forming unit-spleen (CFU-S) of recipient mice were observed. Polymerase chain reaction (PCR) was used to measure Y chromosome (Sry gene) to verify the cell source of hematopoietic reconstruction in receipient mice.
RESULTS AND CONCLUSION: The 30-day survival rate, the number of leucocyte, haematocrit and platelet, the weight of spleen and the number of CFU-S were significantly higher in transplantation group compared with control group (P < 0.05). Y chromosome PCR results indicated that reconstructed hematopoietic cells of female recipient mice were derived from male donor. These suggested that Sca-1+HSC/HPC transplantation can reconstruct hematopoietic function of hematopoietic failure mice.

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