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06 August 2010, Volume 14 Issue 32 Previous Issue    Next Issue

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Proliferation and differentiation of endogenous neural stem cells and brain functional reconstruction following laser irradiation Zhang Wei-hong, Li Wan-qing, Wang Jin-guo, Liu Gui-ping, Yang Hua-shan, Wang Lu-lu 2010, 14 (32):  3077-6080.  doi: 10.3969/j.issn.1673-8225.2010.32.042 Abstract ( 99 )   PDF (255KB) ( 332 )   Save BACKGROUND: Numerous studies have shown that the increase of reproductive activity and self-repair capacity of brain endogenous neural cells might be a valuable method to treat ischemia-hypoxia brain damage. OBJECTIVE: To study the effects of He-Ne laser irradiation on the proliferation and differentiation of endogenous neural stem cells and brain functional reconstruction in newborn rats with hypoxia-ischemia brain damage. METHODS: Newborn rats aged 7 days were prepared for hypoxia-ischemia brain damage models. From the second day of model induction, rats in the laser treatment group were subjected to He-Ne laser irradiation. Acupuncture point included Baihui (DU20) on the median parietal bone, and Dazhui (GV14) between the C7 and T1 and the median back. After the second course, the learning and memory ability of rats were tested by Y-type maze test. Then brain hippocampal sections were made and underwent immunohistochemistry for nestin and microtubule-associated protein-2. RESULTS AND CONCLUSION: In the laser treatment group, the ability of learning and memory were obviously higher than those in the model group (P < 0.05), however, compared with the sham-operated group, the difference was not obviously (P > 0.05). Compared with the sham-operated group, nestin expression in the dentate gyrus was significantly increased in the model and laser treatment groups (P < 0.05), and the increased range was greater in the laser treatment group compared with the model group (P < 0.05). Microtubule-associated protein-2 expression was widely distributed in the cerebral cortex, and darkly stained brown dendrite presented with radiation-shape. Neurons in the hippocampal pyramid and dentate gyrus granular cell layer arranged regularly. Positively stained dendrite presented branch-shape and distributed in the molecular layer. No significant difference was determined between the sham-operated and laser treatment groups. But the microtubule-associated protein-2 expression was significantly weakened in the model group. He-Ne laser irradiation can promote proliferation of endogenous neural stem cells in neonatal rats with hypoxia-ischemia brain damage, induce its differentiation into neurons, thus, achieves reconstruction of learning and memory functions. Related Articles | Metrics

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Platelet-rich plasma induces the differentiation of rabbit bone marrow stromal stem cells into osteoblasts Zhang Li-long, Lu Lei, Zhang Xue-li, Tian Rong 2010, 14 (32):  5891-5896.  doi: 10.3969/j.issn.1673-8225.2010.32.001 Abstract ( 93 )   PDF (462KB) ( 392 )   Save BACKGROUND: The directional induced differentiation of bone marrow stromal stem cells (BMSCs) into osteoblasts has disadvantages of high inducer price, difficult to prepare or long cell culture cycle and low osteogenic ability. Previous studies have confirmed that growth factor in concentrated platelet can induce osteanagenesis. OBJECTIVE: To observe effects of platelet-rich plasma (PRP) on the proliferation and osteoblast differentiation activity of rabbit BMSCs cultured in vitro. METHODS: Bone marrow was taken from the greater trochanter of bilateral femur in 8 rabbits. The rabbit BMSCs were separated and cultivated in vitro. The third generation of BMSCs was divided into two groups. In the experimental group, BMSCs were interfused with DMEM containing 1% PRP, while in the control group, BMSCs were incubated in common DMEM conditioned medium. RESULTS AND CONCLUSION: Under an inverted microscope, the primary cells began to adhere to the wall at 24-36 hours. The cells were confluent at 10 -12 days. Cells in experimental group began to adhere on day 2. Most of the cells were confluent on day 6. The shape change of cells from the control group was later 1-3 days than that of the experimental group. At 2, 6, 10 and 14 days, alkaline phosphatase determination and the calcium node alizarin red staining showed that the rabbit osteobalasts cultured in vitro grew well by this method and the biochemical indexes were stable. They also had the same morphological and biological characters as the osteoblasts. These verified that the PRP can promote the BMSCs proliferation, and can also accelerate effectively the differentiation from BMSCs into osteoblasts. Related Articles | Metrics

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Effects of bone marrow mesenchymal stem cells on nephrin expression in the kidney of adriamycin-induced nephritic rats Dong Chen, Yang Huan-dan, Feng Bing-feng, Guan Feng-jun, Zhao Tong 2010, 14 (32):  5897-5902.  doi: 10.3969/j.issn.1673-8225.2010.32.002 Abstract ( 68 )   PDF (741KB) ( 354 )   Save BACKGROUND: Numerous studies have addressed bone marrow mesenchymal stem cells (BMSCs) transplantation for treating acute kidney injury, but few reports have concerned BMSCs transplantation for chronic kidney disease. OBJECTIVE: To investigate the repair effects of BMSCs transplantation on podocytes of adriamycin-induced nephrotic rats via abdominal aorta and effects on Nephrin mRNA expression in the kidney. METHODS: BMSCs of Sprague Dawley rats were harvested, cultured and purified by whole bone marrow method + adherence screening method, and then used for transplantation. The Sprague Dawley rats were randomly assigned to three groups: normal group: normal Sprague Dawley rats, no intervention; model group: injection of adriamycin once into caudal vein 5 mg/kg + infusion of saline 1 mL into abdominal aorta on the same day; BMSCs group: injection of adriamycin once into caudal vein      5 mg/kg + infusion of stem cells 1×107 into abdominal aorta on the same day, about 1 mL volume. Eight rats from each group were collected at 7, 14 and 28 days following treatment. The cell membrane antigens were detected with flow cytometry analysis. The urine protein was measured at 24 hours using sulfosalicylic acid-sodium sulfate turbidimetric method. Serum albumin and blood fat were examined utilizing automatic biochemistry analyzer. The renal histological changes were observed under transmission electron microscope and using hematoxylin-eosin staining. The expression of Nephrin mRNA in the kidney tissues was measured using reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION: Compared with normal group, rats in model group developed nephropathy characterized by ascites, proteinuria, hypoalbuminemia, hypercholesteremia, and progressive renal injury. However, the symptoms in BMSCs group were significantly ameliorated compared with model group. Nephrin mRNA expression in kidney was significantly decreased in the model group compared with normal group (P < 0.05). Compared with model group, Nephrin mRNA expression was increased in BMSCs group (P < 0.05). Results have suggested that BMSCs play a protective effect in ardriamycin-induced nephrotic rats via increasing nephrin mRNA expression in kidney tissue. Related Articles | Metrics

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Bone marrow mesenchymal stem cells in repairing damaged renal tubular epithelial cells: Possibility and feasibility? Wan Jian-xin, Guo Qi, Pan Yang-bin, Cui Jiong, Fu Bin-bin, Xu Yan-fang 2010, 14 (32):  5903-5907.  doi: 10.3969/j.issn.1673-8225.2010.32.003 Abstract ( 129 )   PDF (405KB) ( 523 )   Save BACKGROUND: Previous studies have suggested that bone marrow mesenchymal stem cells (BMSCs) can directly differentiate into tubular epithelial cells following acute renal failure (ARF) and promote the recovery of renal function. However, the action mechanism of repairing the kidney remains unclear. Whether BMSCs can directly differentiate into tubular epithelial cells is still controversial. OBJECTIVE: To observe the change in renal function in ARF mice after BMSC infusion and to investigate the distribution of exogenous BMSCs in the kidney and the possibility of BMSC differentiation into tubular epithelial cells. METHODS: BMSCs were harvested from green fluorescent protein transgenic mice. A total of 90 healthy female Kunming mice aged 8-10 weeks were randomly assigned to three groups. Mice in the ARF group and BMSC group were injected with cisplatin to establish models of ARF. At 24 hours following cisplatin injection, mice in the BMSC group were injected intravenously with BMSCs of green fluorescent protein transgenic mouse. Normal controls were left intact. At 1, 4, 7, 14 and 28 days after cisplatin injection, the blood urea nitrogen and serum creatinine were measured, and renal morphologic changes were observed, distribution of green fluorescent protein-positive BMSCs in the kidney was examined by fluorescence microscopy. Differentiation of BMSCs into renal tubular epithelial cells was observed using confocal microscopy. RESULTS AND CONCLUSION: After 4-14 days of cisplatin injection, the blood urea nitrogen and serum creatinine value of BMSC group were significantly lower than in ARF group (P < 0.01 or P < 0.05). After 4 days, green fluorescent protein-positive BMSCs were detected in the priopticon area of renal tubule in the BMSC group. On day 7, several green fluorescent protein-positive cells were still detected. Green fluorescent protein-positive BMSCs could express tubular epithelium specific protein megalin. Results have indicated that BMSCs can differentiate into the tubular epithelial cells directly and improve the function of ARF mice. Related Articles | Metrics

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Effect of cell density on differentiation of bone marrow mesenchymal stem cells into neuronal-like cells  Ke Jun-long, Xu Zhi-en, Li Hua, Chen Jing-juan, Liang Wei 2010, 14 (32):  5908-5912.  doi: 10.3969/j.issn.1673-8225.2010.32.004 Abstract ( 108 )   PDF (366KB) ( 356 )   Save BACKGROUND: Cell plant density is one of the factors that affect stem cell differentiation. To date, there is still lack of further study about the effect of cell plant density on bone marrow mesenchymal stem cells differentiation into neuronal-like cells. OBJECTIVE: To investigate the effect of cell density on the differentiation of rat bone marrow mesenchymal stem cells into neuronal-like cells. METHODS: Rat bone marrow mesenchymal stem cells were isolated by use of adherent culture method. Following four passages, cells were cultured in six-well plates at a density of 2×102/cm2, 2×103/cm2, and 4×103/cm2, 8×103/cm2, 2×104/cm2, 4×104/cm2. Cells in each group were differentiated into neuronal-like cells under the induction of basic fibroblast growth factor + epidermal growth factor + RA, and were identified by immunohistochemical staining method. The proportion of neuronal-like cells in each group was calculated, and the differentiation rate was compared. RESULTS AND CONCLUSION: Neuronal-like cells were observed after adding inducer in bone marrow mesenchymal stem cells, and Nestin, neuron-specific enolase, glial fibrillary acidic protein staining was positive. Different cell plant densities induced different ratios of neuron-like cells. 8×103/cm2 plant density yielded highest proportion of neuronal-like cells and can survive as long as 7 days. The results demonstrated that the differentiation of bone mesenchymal stem cells into neuron-like cells was correlated with cell plant density. Too high or too low cell plant density was detrimental to differentiation. Related Articles | Metrics

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Adrenomedullin gene transfection improves anti-apoptotic ability of bone marrow mesenchymal stem cells under hypoxic conditions Cai Wen-qin, Su Jin-zi, Yu Deng-ji 2010, 14 (32):  5913-5917.  doi: 10.3969/j.issn.1673-8225.2010.32.005 Abstract ( 94 )   PDF (334KB) ( 380 )   Save BACKGROUND: Gene transfection can elevate the survival of bone marrow mesenchymal stem cells (BMSCs) in transplanted region, but there are a few reports addressing proliferation, apoptosis and secretion of adrenomedullin (ADM) gene-transfected BMSCs under hypoxic condition. OBJECTIVE: To observe effects of ADM gene transfection on BMSCs proliferation, apoptosis and secretion of vascular endothelial growth factor under hypoxic and serum-free culture conditions. METHODS: Rat BMSCs were isolated and amplified in vitro by differential adhesion method. The infection efficiency of recombinant adenovirus Ad-ADM in BMSCs was tested by x-gal staining. BMSCs were divided into non-transfection group, empty vector group and Ad-ADM transfection group. BMSCs cultured under hypoxia and serum-free conditions for 0, 3, 6, 9, 12, 16, 20, 24 hours respectively. Cell proliferation was detected by cell counting kit-8 assay. ADM and vascular endothelial growth factor in culture medium were detected by enzyme-linked immunosorbent assay. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick labeling. RESULTS AND CONCLUSION: Infection rate of BMSCs increased with multiplicity of infection (MOI) in a dose-dependant manner. When MOI was 150, the infection rate was 95.4%. After BMSCs cultured in hypoxia and serum-free medium, cell growth inhibition and apoptosis increased, but the apoptotic rate in Ad-ADM transfection group was significantly lower than other 2 groups after cultured for 0, 3, 6, 9, 12, 16 hours (P < 0.05). At 9 and 12 hours, BMSCs secreted ADM and vascular endothelial growth factor reached a peak and were significantly higher in the Ad-ADM transfection group than other two groups (P < 0.05). Results have indicated that under hypoxia and serum-free culture conditions (< 20 hours), ADM gene transfection enhances the anti-apoptotic ability of BMSCs, which may be associated with the increased expression of ADM and vascular endothelial growth factor. Related Articles | Metrics

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Cocultivation of human cord blood mononuclear cells and gastric cancer cells: Screening of optimal effector/target ratio Wu Shi-kai, Yang Bo, Du Ying, Hu Xiang, Zheng Yi-duan, Ning Xiao-lin 2010, 14 (32):  5918-5922.  doi: 10.3969/j.issn.1673-8225.2010.32.006 Abstract ( 88 )   PDF (452KB) ( 434 )   Save BACKGROUND: Many research pay attention to umbilical cord blood cells can inhibit tumor cells in recent years, people tried to suppress cancer cells by umbilical cord blood cell transplantation and these treatments may provide a new way for curing cancer. OBJECTIVE: To investigate the effect of adhered mononuclear cells from human cord blood and gastric cancer cells after co-cultured in vitro. METHODS: Cord blood mononuclear cells were separated by density gradient centrifugation, and the expression of surface marker of the mononuclear cells was detected by flow cytometry. Then the adhered cells and gastric cancer cells were co-cultured, and cell morphology were observed under an inverted microscope, and lactate dehydrogenase (LDH) detection was used to identify anti-tumor efficacy of cord blood mononuclear cells and to screen best effector/target ratio. RESULTS AND CONCLUSION: At 2 days after accession of cord blood mononuclear cells, the shedding of tumor cells was obviously. In the experimental group, the tumor cells were arranged irregularly with low cell density. LDH method showed that some adherent cells from umbilical cord blood have strong anti-tumor capabilities, but the anti-tumor capacity in different individuals were varied in 4-fold. Adherent cells from umbilical cord blood have strong killing effect on tumor cells, and best anti-target ratio was 2:1. Related Articles | Metrics

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Effect of human bone marrow stromal cells in combination with several cytokines on in vitro amplification of mononuclear cells derived from umbilical cord blood   Xu Li, Mao Ping 2010, 14 (32):  5923-5926.  doi: 10.3969/j.issn.1673-8225.2010.32.007 Abstract ( 87 )   PDF (243KB) ( 327 )   Save BACKGROUND: The goal of in vitro amplification of umbilical cord hematopoietic cells is promoting the engrafting capacity of umbilical cord hematopoietic cells, and the cytokine mediating can accelerate cell amplification, which exhausted the hemopoietic stem cells simultaneously.    OBJECTIVE: To explore the effect of human bone marrow stromal cells (BMSCs) in combination with several cytokines on the in vitro expansion of the mononuclear cells derived from umbilical cord blood and the expression of adhesion molecule and CXCR4 after cell expansion. METHODS: The isolated human mononuclear cells were incubated on BMSCs layers and cultured in 4 groups. Cells in the control group were cultured in single mononuclear cells; those in the other groups were cultured in several cytokines + mononuclear cells, BMSCs+ mononuclear cells, BMSCs+ several cytokines, and several cytokines + mononuclear cells, respectively. At 0 and 7 days after culture, the number of total nucleated cells, CD34+ cells, CD34+CXCR4+ , CD34+CD49d+ , and CD34+CD62L+ cells were counted. RESULTS AND CONCLUSION: The single BMSCs and cytokine could effectively promote the amplification of umbilical cord hematopoietic cells and increased the expressions of CD49d, CD62L and CXCR4. The cell multiplication ability in the single cytokine group was greater than that in the BMSCs group, but poorly increased the expressions of CD49d, CD62L and CXCR4. It revealed that BMSCs can support the amplification of umbilical cord hematopoietic cells, but the supporting ability is limited. However, BMSCs in combination with several cytokines can effectively promote amplification of umbilical cord hematopoietic cells, the results of which is superior to the single cytokine or BMSCs. The results demonstrated that BMSCs in combination with several cytokines can enhance expansion of cord blood mononuclear cells, and promote the adhesion and chemotactic capacity of umbilical cord hematopoietic cells. Related Articles | Metrics

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Inhibitory effects of human umbilical cord blood-derived mesenchymal stem cells on hypoxia-induced cardiomyocyte apoptosis Huang Jing-ling, Yang Shui-xiang, Chen Yi-rong 2010, 14 (32):  5927-5930.  doi: 10.3969/j.issn.1673-8225.2010.32.008 Abstract ( 104 )   PDF (370KB) ( 308 )   Save BACKGROUND: Anti-apoptosis is a new direction of bio-therapy of heart failure. Recent studies have found that adult mesenchymal stem cells (MSCs) under certain conditions can be induced to differentiate into cardiomyogenic cells. By secreting a variety of cytokines, MSCs can promote cardiac angiogenesis and reduce cardiomyocyte apoptosis. OBJECTIVE: To investigate the anti-apoptotic effects of human umbilical cord blood (UCB)-derived MSCs on hypoxia-induced human cardiomyocytes apoptosis. METHODS: Following resuscitation, human cardiomyocytes were incubated in a 6-well plate (control group) and Transwell 3412 plate. Human UCB-derived MSCs were incubated on Transwell-inserted permeable filter membrane. Cells in both groups were incubated in 95% N2+ 5% CO2 hypoxia condition for 2, 4, 12 and 24 hours. Apoptotic rate of cardiomyocytes was detected in both groups. The level of insulin-like growth factor-1 (IGF-1) in the conditioned medium was measured with enzyme linked immunosorbent assay kit. RESULTS AND CONCLUSION: From the third passage, human UCB-derived MSCs and primary culture of cardiomyocytes had IGF, but IGF-1 levels were significantly greater in human UCB-derived MSCs conditioned medium than human cardiomyocytes medium. Hypoxia can induce cardiomyocyte apoptosis, and human UCB-derived MSCs exerted protective effects on hypoxia-induced cardiomyocyte apoptosis under short-term and persistent hypoxia. Cardiomyocyte apoptotic rate was lower in the human UCB-derived MSCs group than control group (P < 0.05). The results have demonstrated that human UCB-derived MSCs exhibit significant anti-apoptotic effects on hypoxia-induced human cardiomyocytes. This protective effect may be associated with cell to cell direct interaction and paracrine of cytokine. Related Articles | Metrics

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Mild hypothermia effects on migration of bone marrow mesenchymal stem cells in rats with cerebral ischemia  You Hui,Peng Yu,Zhang Qi-mei,Li Cheng-yan 2010, 14 (32):  5931-5934.  doi: 10.3969/j.issn.1673-8225.2010.32.009 Abstract ( 86 )   PDF (319KB) ( 305 )   Save BACKGROUND: There are a few reports addressing application of mild hypothermia to the study of repairing nerve injury, but few reports have addressed mild hypothermia effects on migration of neural stem cells in the brain following transplantation. OBJECTIVE: To explore the effects of mild hypothermia on migration of BMSCs transplanted into lateral ventricle of rats after cerebral ischemia. METHODS: A focal cerebral ischemic injury model of right middle cerebral artery occlusion (MCAO) was established using modified Longa’s method in Sprague Dawley rats. Local mild hypothermia was applied in the mild hypothermia group prior to transplantation. Normal body temperature was maintained in control group before transplantation. At 24 hours following model establishment, BMSCs labeled with 5-BrdU were transplanted into the rat lateral ventricle under brain stereotaxis. The amount of BrdU-positive cells was measured by immunohistochemistry at 5, 14, 21, 28 days and 7 weeks following injection in each group. RESULTS AND CONCLUSION: At 14 days after transplantation, a majority of labeled BMSCs had migrated to the infarct area. At various time points following transplantation, the number of BrdU-positive cells was obviously greater in the hypothermia group compared with the control group (P < 0.05). Results have indicated that mild hypothermia may promote the migration of BMSCs, and shows neuroprotective effects on focal cerebral ischemia. Related Articles | Metrics

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Differences in neural stem cells proliferation between normal senescence and senescence-accelerated mice Liu Jie, Yin Hai-yan, Yu Neng-wei, Qiao Xiu-lan, Lu Sheng-feng, Zeng Fang, Huang Mei, Wei Jiao-lu, Tang Yong 2010, 14 (32):  5935-5938.  doi: 10.3969/j.issn.1673-8225.2010.32.010 Abstract ( 94 )   PDF (433KB) ( 459 )   Save BACKGROUND: In the aging process, the changes of environment in the brain can cause changes in proliferation ability of brain neural stem cells. The neural stem cells are closely associated with senescence and neurodegenerative diseases. Proliferation ability is negatively correlated with age. However, no reports have addressed that senescence-accelerated mice served as senescence models. OBJECTIVE: To investigate the differences of neural stem cells proliferation between normal senescence and senescence-accelerated mouse in hippocampus, olfactory bulb and cortex. METHODS: The hippocampus, olfactory bulb and cortex were obtained from 6 senescence-accelerated mice (senescence-accelerated mouse prone 8) and 6 normal senescence mice (senescence-accelerated mouse/resistance 1). Following frozen section, Ki-67/Nestin immunofluorescence double labeling methods were used to detect the proliferation of neural stem cells in hippocampus, olfactory bulb and cortex. Pictures of immunofluorescence double labeling were taken through Leica Qwin v3 under a fluorescence microscope. Using 40× object lens and 10× eyelens, five consecutive visual fields were selected from each section, and image analysis was conducted using Image-pro-Plus software. RESULTS AND CONCLUSION: The proliferation of neural stem cells could be found both in normal senescence and senescence-accelerated mice, but there were differences, mainly in the hippocampus and olfactory bulb (P < 0.05). Results indicated that senescence-accelerated might result in low ability of neural stem cells proliferation in hippocampus and olfactory bulb. Related Articles | Metrics

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Isolation, culture and differentiation of amnion mesenchymal stem cells into neuron-like cells in vitro  Jin Jun, Huang Jian, Wang Jun, Fu Jian-hong 2010, 14 (32):  5939-5943.  doi: 10.3969/j.issn.1673-8225.2010.32.011 Abstract ( 74 )   PDF (578KB) ( 409 )   Save BACKGROUND: Placenta as a new source of seeking mesenchymal stem cells has been paid great attention. To harvest cells from amnion layer of placenta has wide application prospects due to its advantages, such as extensive source and no ethical limitation. OBJECTIVE: To study the method of in vitro isolating human amnion mesenchymal stem cells, and the ability of differentiating into neuron-like cells. METHODS: Mesenchymal stem cells were harvested and cultured from amnion by enzyme digestion method. The surface marker and purity of human amnion mesenchymal stem cells were measured by uniform morphology and flow cytometry. The human amnion mesenchymal stem cells were induced to differentiate using DMEM-LG+20 g/L DMSO+100 µmol/L BHA+ 10 μg/L basic fibroblast growth factor. The differentiated cells were identified with immunofluorescence staining. RESULTS AND CONCLUSION: Mesenchymal stem cells could be successfully harvested from amnion. Cells grew and adhered to the wall, and could proliferate and subculture in a short period in vitro. Amnion mesenchymal stem cells had similar surface markers to bone marrow mesenchymal stem cells, and could differentiate into neuron-like cells in vitro. Amnion mesenchymal stem cells were positive for glial fibrillary acid protein and neuron specific enolase. Results have suggested that amnion mesenchymal stem cells could be induced to differentiate into neuron-like cells and amnion tissue could be a novel source in stem cells study and for nerve tissue diseases. Related Articles | Metrics

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Isolation of human placenta derived mesenchymal stem cells by enzymatic digestion versus global perfusion Li Fang, Miao Zong-ning, Xu Yun-yun, Zhang Xue-guang 2010, 14 (32):  5944-5948.  doi: 10.3969/j.issn.1673-8225.2010.32.012 Abstract ( 113 )   PDF (448KB) ( 337 )   Save BACKGROUND: The method of isolation and purification of human placenta derived mesenchymal stem cells (PMSCs) has certain blindness due to the interference of other cells within the placenta. OBJECTIVE: To investigate the efficiency of two methods to isolate PMSCs such as enzyme digestion and global perfusion, to find an efficient method and to establish a stable in vitro culture system of PMSCs. METHODS: The normal term placenta was obtained to isolate PMSCs by different methods. Ⅳ collagenase digestion was used in the enzymatic digestion group, and global perfusion was utilized in the global perfusion group. The growth characteristics of cells, cell surface marker, as well as the multiple differentiation potential of the cultured cell obtain by these two methods were compared following conventional culture. RESULTS AND CONCLUSION: There was no significant difference of the cell growth, multiple differentiation potential between the cultured cell obtain by these two method mentioned above, but the CD90 was obviously lowly expressed on the cultured cells obtained by enzymatic digestion compared with global perfusion (P < 0.05), which shows that global perfusion is a more efficient way to get higher purity of PMSCs compared with enzymatic digestion. Related Articles | Metrics

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Differentiation of human placenta-derived mesenchymal stem cells into cholinergic neuron-like cells in vitro  Jing Xu-dong, He Dong-mei, Zhang Yuan, Chen Li 2010, 14 (32):  5949-5951.  doi: 10.3969/j.issn.1673-8225.2010.32.013 Abstract ( 68 )   PDF (447KB) ( 309 )   Save BACKGROUND: Human placenta may contain similar mesenchymal stem cells (MSCs), which can be differentiated into cholinergic neuron-like cells. OBJECTIVES: To explore the differentiation of MSCs from human placenta into cholinergic neuron-like cells in vitro. METHODS: MSCs from human placenta were isolated and purified by cell culture. The fourth passage of MSCs was incubated with fetal bovine serum+basic fibroblast growth factor (bFGF) for 2 days, and divided into 3 groups: groupⅠ, MSCs were incubated with DMEM/F12 complemented with bFGF, 2-mercaptoethanol, retinoic acid and nerve growth factor for 19 days. GroupⅡ, based on Group Ⅰ, epidermal growth factor and heparin were added into the culture system for 19 days. In the negative control group, cells were cultured by DMEM/F12 containing fetal bovine serum. RESULTS AND CONCLUSION: The adhesive cells gradually formed flat monolayer cells, parallel arranged or whirlpool grew, with uniform morphology after 2 passaged. The fourth passage of MSCs from human placenta was strongly positive for CD44, CD29, but negative for CD34, CD45, CD106 and HLA-DR. After 2 weeks of culture, nestin, chat mRNA could be expressed. The positive rate of nestin, Ache and chat mRNA was higher in the group Ⅰ and group Ⅱ than that of the negative control group (P < 0.05). Compared to the negative control group, the content of acetylcholine in supernate was obviously increased in the group Ⅰ and group Ⅱ (P < 0.05), especially more in the group Ⅰ (P < 0.05). The results demonstrated that MSCs from human placeta can differentiate into cholinergic neuron-like cells, which are able to synthesis, release and disintegrate acetylcholine. Related Articles | Metrics

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Isolation, culture and identification of menstrual blood-derived mesenchymal stem cells Zhou Yun-fan, Yang Bo, Hu Xiang, Jiao Hong-liang, Zhou Chang-hui, Tian Yi, Lei Ning-jing, Gu Chen-xi, Xu Yu-ming, Dai Jian-wu, Guan Fang-xia 2010, 14 (32):  5952-5956.  doi: 10.3969/j.issn.1673-8225.2010.32.014 Abstract ( 258 )   PDF (438KB) ( 934 )   Save BACKGROUND: Generally, the main sources of stem cells are embryonic stem cells and bone marrow stem cells, but there are some limitations of the two kinds of sources, and researchers strive to find a new type of stem cells in order to overcome the potential for teratoma, the lack of sample sources, ethics controversies and so on. OBJECTIVE: To isolate, culture and identify menstrual blood-derived mesenchymal stem cells (MMCs). METHODS: MMCs were separated by Ficoll density gradient centrifugation. Cell morphology and growth characteristics were observed. Flow cytometry analysis was used to identify expression of CD29, CD44, CD34, CD45, HLA-ABC and HLA-DR surface markers in MMCs. Immunocytochemical method was utilized to detect nestin-positive cells in MMCs. RESULTS AND CONCLUSION: MMCs were successfully separated from menstrual blood by Ficoll density gradient centrifugation. About 2 weeks later, primary cells reached 80% to 90% confluence; cells were spiral-shaped, mesh and radial. After passage to a stable growth, cells could be seen as the leading fiber-like cell morphology. Results from flow cytometry showed that MMCs were highly positive for CD29, positive for CD44, low positive for HLA-ABC, but negative for CD34, CD45 and HLA-DR. Staining showed nestin expression of antigen in MMCs was about (10.35±0.51)%. Results have suggested that MMCs express MSCs markers, and only have low immunogenicity. MMCs express nestin antigen and provide some theoretical basis for MMCs in the nervous system application. Related Articles | Metrics

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Duhuojisheng decoction combined with bone marrow stromal stem cells complex in repair of articular cartilage defects in rabbits  Li Chun-gen, Kang Xiao-le, Song Guang-ming, Li Xiao-ping 2010, 14 (32):  5957-5961.  doi: 10.3969/j.issn.1673-8225.2010.32.015 Abstract ( 113 )   PDF (384KB) ( 342 )   Save BACKGROUND: Bone marrow stromal stem cells (BMSCs) combined with acellular dermal matrix (ADM) can repair the articular cartilage defects in rabbits. Chinese medicine Duhuojisheng soup can promote cartilage repair, and improve the quality of repair. OBJECTIVE: To further verify Duhuojisheng decoction effects on the repair of rabbit articular cartilage defects using BMSCs complex. METHODS: Rabbit BMSCs following in vitro proliferation were compounded on ADM, which was then implanted into rabbit knee cartilage defects. Traditional Chinese medicine Duhuojisheng decoction was given, which served as a Duhuojisheng decoction + stem cell group. This study also set Duhuojisheng decoction group, composite grouped and blank group (control group). Morphological observation and histological examination were performed in repaired tissue at 4, 8, 12 weeks. RESULTS AND CONCLUSION: After 12 weeks, the defect in the Duhuojisheng detection + stem cell group was filled with hyaline cartilage-like tissue; cartilage and subchondral bone tissue were basically repaired; repair of cartilage in histomorphology was superior to other groups. Results have indicated that BMSCs complex can repair articular cartilage defects in rabbits, and Chinese medicine Duhuojisheng decoction can promote cartilage repair, and improve the quality of repair. Related Articles | Metrics

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Long-term follow-up after allogeneic hematopoietic stem cell transplantation Dong Min, Wu Xiang-yuan, Lin Qu 2010, 14 (32):  5962-5966.  doi: 10.3969/j.issn.1673-8225.2010.32.016 Abstract ( 272 )   PDF (296KB) ( 395 )   Save BACKGROUND: With the rapid development of hematopoietic stem cell transplantation (HSCT) in China over the last 20 years, more and more patients have been cured after allogeneic HSCT; however, few reports discussed the long-term follow-up results of post-transplantation. OBJECTIVE: To summarize a long-term follow-up after allogeneic HSCT. METHODS: There were 30 patients who received allogeneic HSCT at the Third Affiliated Hospital of Sun Yat-sen University between 1995 and 2005. We summarized patients’ long-term complications, survival rate and cause of death and investigated quality of life of long-term survival patients. RESULTS AND CONCLUSION: In 30 cases, the incidence of chronic graft versus host disease was 67%. Each patient suffered from infertility, and one patient had slight pulmonary fibrosis. No other complications were detected. Disease-free survival two years after transplantation was 60%. Patients without relapse could survive for a long time within two years. The longest surviving time was over 14 years. 12 of 30 patients died, and all of those deaths occurred in two years post-transplantation. Five cases died of recurrence, and other seven patients died of infection. Long-term survival patients had poor life quality one year after transplantation, and their life quality gradually improved with prolonged survival time. The quality of life was improved significantly five years post-transplantation, and the quality of life was high at eight years. The main influential factor for quality of life was chronic graft versus host disease. Related Articles | Metrics

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Stem cells for treatment of autism: Safety and efficacy Liu Min, Sun Liang-wei, Lü Yong-tao, Huan Ying, Ge Ru-cun, Cao Yu-lin, Guo Chuan-qin, Chen Xing-wang 2010, 14 (32):  5967-5970.  doi: 10.3969/j.issn.1673-8225.2010.32.017 Abstract ( 216 )   PDF (277KB) ( 719 )   Save BACKGROUND: Autism is associated with several neurophysiological alterations, especially immune abnormalities and neural hypoperfusion appear to be broadly consistent. OBJECTIVE: To evaluate the feasibility, safety and efficacy of stem cells for treating autism. METHODS: A total of 42 children of autism were divided into three groups: umbilical cord blood group, combination group and control group. Umbilical cord blood group was treated with umbilical cord blood mononuclear cells. Combination group was treated with umbilical cord blood mononuclear cells combined with umbilical cord mesenchymal stem cells. Control group received rehabilitation treatment. Related lab examinations were taken for umbilical cord blood and combination groups before treatment, 4 weeks after treatment and 4 months after treatment. Whether there were adverse reactions were observed. Childhood Autism Rating Scale and Clinical Global Impression Scale were employed to assess the children of autism before treatment, 4 weeks and 4 months following first treatment in patients of each group. RESULTS AND CONCLUSION: There was no statistical difference in lab examinations between umbilical cord blood and combination groups before treatment, 4 weeks and 4 months following treatment. There were no severe adverse reactions following stem cell treatment. Childhood Autism Rating Scale was significantly decreased in the umbilical cord blood and combination groups before treatment, 4 weeks and 4 months following treatment. Clinical Global Impression Scale demonstrated that total efficiency was greater in the combination group compared with umbilical cord blood group. Results indicated that it is safe to use umbilical cord blood mononuclear cells and umbilical cord mesenchymal stem cells for treating children autism and the therapeutic effect of the umbilical cord blood and combination groups was noticeably higher than that of the control group with rehabilitation treatment. Related Articles | Metrics

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Safety of intravenous transplantation of heterogenous umbilical cord mesenchymal stem cells Zhang Xue-feng, Zhang Mei-rong, Hu Jian-xia, Wang Li, Zhang Gui-zhi, Shao Lei, Li Sheng-gang, Gao Hong, Miao Zhi-min, Wang Yan-gang 2010, 14 (32):  5971-5975.  doi: 10.3969/j.issn.1673-8225.2010.32.018 Abstract ( 135 )   PDF (410KB) ( 542 )   Save BACKGROUND: Human umbilical cord mesenchymal stem cells (HUMSCs) can be used as cells for tissue repair and regeneration engineering due to its abundant source, strong proliferation and differentiation ability, uninvolved to ethics problems. However, few studies have addressed the safety of heterogenous HUMSC transplantation via intravenous injection. OBJECTIVE: To study the safety of intravenous injection of HUMSCs in Wistar rats. METHODS: A total of 30 SPF Wistar rats were equally and randomly divided into three groups: blank control, negative control and HUMSC transplantation groups, of equal gender. HUMSCs were isolated and cultured in vitro. Rats in each group were injected via tail vein, with 1 mL of 5×106 HUMSCs as HUMSC transplantation group and 1 mL of 50 g/L glucose as negative control. The rats were observed for 14 days for their general reactions before they were sacrificed. Blood samples were collected for hematological and biochemical studies. Visceral organs were inspected, weighted and studied pathologically. RESULTS AND CONCLUSION: No significant difference in hematological and biochemical studies was detected between HUMSC transplantation group and control group (P > 0.05). No significant difference was determined in histopathological observation in the viscera organs between HUMSC transplantation group and control group. Results have suggested that intravenous transplantation of heterogenous HUMSCs is safe and feasible. No side effects were found in recipients. Related Articles | Metrics

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Migration, proliferation and differentiation of spermatogonial stem cells in vivo after transplantation Ma Liang-hong, Ding Qiang, Feng Li-xin, Li Pei-jun, Chen Fu-bao 2010, 14 (32):  5976-5982.  doi: 10.3969/j.issn.1673-8225.2010.32.019 Abstract ( 196 )   PDF (602KB) ( 358 )   Save BACKGROUND: Spermatogonial stem cell transplantation has potential clinical value for the treatment of male infertility. However, the process of stem cell migration, proliferation and differentiation in vivo is not yet entirely clear. OBJECTIVE: To observe the migration, proliferation and differentiation of spermatogonial stem cells in vivo after transplantation.  METHODS: Using C57BL/6 mice of postnatal 6-10 days as the germ cell donors, and male germ cells were obtained by combination with compound enzymatic digestions, differential velocity adherent technique and discontinuous Percoll density gradient centrifugation. Male C57BL/6 mice of postnatal 6 weeks were used as the recipients. They had been injected busulfan intraperitoneally, which could destroy endogenous spermatogenic function. In the experimental group, spermatogonial stem cells from donors were infused into the receptor testis using seminiferous tubule microinjection. We traced the PKH26-GL fluorescent-labeled cells in the recipient testes after transplantation. The migration process was observed in vivo. Western blot and RFQ-PCR were utilized to measure α6-Integrin, c-kit, SCF protein and mRNA changes in testis tissues. Normal mice without receiving busulfan treatment and cell transplantation were selected as the parallel positive control group. Mice which were microinjected media into seminiferous tubules in unilateral testes served as the parallel negative control group. RESULTS AND CONCLUSION: Tracing the PKH26-GL fluorescent-labeled cells, partial transplanted cells immigrated into basal membrane of the seminiferous tubules at one week after transplantation. At one month after transplantation, these cells had immigrated into the basal membrane and had division growth. At 3 months, a large number of spermatid formed in the seminiferous tubule. At 1, 2 and 3 months, the expression level of α6-Integrin and c-kit protein was increased gradually in each group (P < 0.01). In negative control and experimental groups, SCF protein expression levels were increased gradually (P < 0.05). α6-Integrin, c-kit and SCF mRNA expression had increased tendency in each group (P < 0.05). Results have suggested that after high-dose chemotherapy, there are a certain number of Sertoli cells in the seminiferous epithelium. This microenvironment of spermatogenesis is not been destroyed completely. Following transplantation, spermatogonial stem cells can proliferate and differentiate in microenvironment provided by recipient Sertoli cells. Related Articles | Metrics

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Establishment of an in vitro culture system of ectomesenchymal cells isolated from the first branchial arch   Yan Zheng-bin, Hou Jing-qiu, Yan Xue-dan, Wang Tian-xiang, Liu Yi-song 2010, 14 (32):  5983-5987.  doi: 10.3969/j.issn.1673-8225.2010.32.020 Abstract ( 92 )   PDF (304KB) ( 306 )   Save BACKGROUND: During the dentofacial development, the first branchial arch as a transitional structure plays an important role. At present, domestic and international research data for the first branchial arch mesenchymal cells were relatively few. OBJECTIVE: To isolate and culture the mesenchyme cells from the first branchial arch of the mice embryos. METHODS: The first branchial arch primordia from E9.5 embryos were dissected under a microscope. Explanted culture and improved enzyme digestion were used to primarily culture. The growth curve, population doubling time, cells proliferation and morphology were studied and specific markers were detected to study biological characteristics of cells. RESULTS AND CONCLUSION: The cells were grown to confluency for 7-10 days, with abundant epithelial cells. Improved enzyme digestion of primary culture had less epithelial-like cells, the cells were grown to confluency for 2-3 days. The growth curve, population doubling time and morphology were similar in both groups after passage. Anti-CD57/HNK-1 and anti-vimentin were all positive. Results have indicated that sufficient and high purity ectomesenchymal cells could be harvested in short time by improved enzyme digestion. Related Articles | Metrics

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Isolation and identification of cancer stem cells in lung cancer cell lines as well as function analysis Wu Jie, Liu Jing, Wang Xiu-dong, Zhu Ling-yun, Wu Zu-ze,Yu Ai-ping 2010, 14 (32):  5988-5991.  doi: 10.3969/j.issn.1673-8225.2010.32.021 Abstract ( 87 )   PDF (336KB) ( 334 )   Save BACKGROUND: It is difficult to maintain the “stem cell” characteristics of cancer stem cells in vitro during proliferation and culture. To obtain cancer stem cells from cancer cell lines that can be kept for a long time is a reliable means. OBJECTIVE: To investigate whether cancer stem cells exist or not in lung cancer cell strains with similar genetic background and different metastasis potential and function thereof. METHODS: The adhesion assays on rat collagen or fibronectin were used to assess the metastasis potential of PLA-801D and PLA-801C. The existence of cancer stem cells in the two cell strains was demonstrated with the differentiation potency of their single cell colons and subclones. The biomechanical remodeling potential of the two cell strains was evaluated with collagen gel contraction assays. RESULTS AND CONCLUSION: Compared with PLA-801C cells, PLA-801D cells showed significantly stronger adhesion on both rat collagen and fibronectin (P < 0.01). PLA-801D cells could form four kinds of single cell clones, and one of them could differentiate into the same four clones in subclones. However, only three kinds of clones were found in PLA801C cells, and none of them could differentiate into the initial three clones in their subclones. PLA-801D cells showed better collagen gel contraction than PLA-801C cells (P < 0.05). These results indicate that a grouplet of cells in PLA-801D cells show self-renewal and multi-directional differentiation characteristic of cancer stem cells. Moreover, PLA-801D cells display good biomechanical remodeling potential, indicating its good remodeling capacity for extracellular matrix. Related Articles | Metrics

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Remnant lipoprotein induces adipogenic differentiation of rat adipose tissue-derived mesenchymal stem cells Ma Mei-fang, Zhang Qian, Liu Ling 2010, 14 (32):  5992-5996.  doi: 10.3969/j.issn.1673-8225.2010.32.022 Abstract ( 62 )   PDF (469KB) ( 349 )   Save BACKGROUND:Very low density lipoprotein and chylomicron are increased in blood plasma of patients with hypertriacylglycerolemia, and can be hydrolyzed into remnant lipoprotein. Very low density lipoprotein can induce the adipogenic differentiation of other preadipocytes. It is presumed that remnant lipoprotein also can induce the adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (ADMSCs). OBJECTIVE:To explore the effects of different concentrations of remnant lipoprotein on the adipogenic differentiation of ADMSCs. METHODS: Collagenase type II was used to isolate ADMSCs from inguinal adipose tissue of rats under sterile condition. The cells were cultured in the DMEM/F12 medium supplemented with fetal bovine serum. The morphological changes of ADMSCs were observed. Flow cytometry was performed to determine cell surface molecules. The second passage of ADMSCs was divided into four groups. Cells in the control group were incubated in 10 mg/L insulin. 50, 100, 200 mg/L remnant lipoprotein was added in the other three groups. Lipid droplets within cells were identified by Oil red O staining 18 days later. RESULTS AND CONCLUSION:  Primary ADMSCs grew in a manner like colony. With the increased passage, ADMSCs looked like spindle fibroblasts and were positive for CD105 but negative for CD34. Compared with the control group, no significant difference in the strength of Oil red O staining was detected following an addition of 50 mg/L remnant lipoprotein (P > 0.05), and the strength of Oil red O staining was significantly increased following an additional 100 mg/L remnant lipoprotein (P < 0.05). The strength was significantly greater in 200 mg/L remnant lipoprotein group than other three groups (P < 0.05). Results have suggested that remnant lipoprotein induces adipogenic differentiation of ADMSCs in a concentration dependent manner. Related Articles | Metrics

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Isolation, culture and identification of human adipose tissue-derived mesenchymal stem cells Yin Li-bo, Zhao Wen-xiu, Yin Zhen-yu, Yang Zhong-meng, Zhang Qiong, Wang Xiao-min 2010, 14 (32):  5997-6000.  doi: 10.3969/j.issn.1673-8225.2010.32.023 Abstract ( 118 )   PDF (487KB) ( 707 )   Save BACKGROUND: Human adipose tissue-derived mesenchymal stem cells (hADSCs) can differentiate toward mesoblast cell lineage under certain conditions, such as the osteogenic, chondrogenic, myogenic and hepatic lineages, and also can differentiate toward epiblastic and endoblastic layers. Those studies demonstrated that the hADSCs have the capacity of multidifferentiation. OBJECTIVE: To study isolation and identification of hADSCs. METHODS: Human adipose tissue was collected after liposuction surgery from the cosmetic and plastic surgery center, digested with 0.075 % collagenase I. Adherent culture could obtain hADSCs. Cell proliferation curve was drawn, and flow cytometry, cell cycle test and immunofluorescence test were conducted to verify whether hADSCs have multiple functional differentiation potential. RESULTS AND CONCLUSION: hADSCs exhibited fibroblast-like morphology, well proliferation, with obvious exponential growth phase, and could survive in vitro for a long time and maintain a undifferentiated state.  Flow cytometry has shown that cells were highly positive for CD90, CD105, and weakly positive for CD34 and CD45,almost 80% cells stayed in resting state(G0/G1). Cells could differentiate into osteogenic and adipogenic direction. Results have indicated that isolated stem cells show the typical characterization of hADSCs, which is a basis for further studying hADSCs. Related Articles | Metrics

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Proliferation of vascular endothelial cells and adipose tissue-derived stem cells under in vitro coculture Wang Fu-ke, Liu Liu, He Xiao-guang, Zhao De-ping, Yuan Rui-hong, Dai Xiao-ming, Li Yi-song 2010, 14 (32):  6001-6005.  doi: 10.3969/j.issn.1673-8225.2010.32.024 Abstract ( 72 )   PDF (431KB) ( 291 )   Save BACKGROUND: Vascular endothelial cells (VECs) have the ability to enhance adipose tissue-derived stem cells (ADSCs) to ossify and new blood vessel can provide nutrition for the ossification of stem cells. OBJECTIVE: To explore the influence of coculture of VECs and ADSCs on cell proliferation in vitro. METHODS: We set simple VECs group, 3: 1, 1: 3, 1: 1 coculture group and simple ADSCs group. Cells were incubated in L-DMEM containing fetal bovine serum at 37 ℃ in a 5% CO2 saturated humidity incubator. Morphological changes were observed by inverted microscope. Cell growth curve was drawn using modified MTT assay. RESULTS AND CONCLUSION: ADSCs and VECs were mixed at 3: 1 and 1: 1 for 14 days. Many synaptic linkages were visible among cells. Partial cells fused into a mass. Cell masses were not observed by other culture methods. With prolonged time, the absorbance of every group was increased gradually and reached a peak in ADSCs, 3: 1, 1: 3 and 1: 1 ratio groups on day 12 and the highest was the 1:1 group, but the time of reaching the peak was on day 10 in the VECs group. The absorbance was gradually reduced under other culture methods. Results have indicated that VECs and ADSCs can stimulate proliferation each other in the system of co-culture in vitro; it can reach the best result when the proportion is 1: 1. Related Articles | Metrics

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Honey for treatment of diabetic wounds and mobilization of endothelial precursor cells Li Lan, Lu Zu-qian, Li Xiang, Ji Qiu-he, Xu Zhang-rong, Cao Ming-jun 2010, 14 (32):  6006-6009.  doi: 10.3969/j.issn.1673-8225.2010.32.025 Abstract ( 146 )   PDF (360KB) ( 527 )   Save BACKGROUND: New vessel is a basic process of wound healing, and bone marrow-derived endothelial progenitor cells (BM-EPCs) play a key role in this condition. It can contribute to form new vessels in ulcer area that BM-EPCs mobilize from bone marrow to peripheral blood circulation and migrate to ulcer wound. Stromal cell-derived factor-1α (SDF-1α) is on e key molecule, which regulates this migration of the precursor cells of new vessels to damaged tissue. OBJECTIVE: To investigate mobilization of endothelial progenitor cells following honey treatment and the effects of honey on SDF-1α expression. METHODS: A round full-thickness skin wound of 1.8 cm diameter was created on the backs of streptozotocin (STZ) induced diabetic rats. The rats were randomized into two groups: saline group and honey group. The wound surface was observed. The number of endothelial progenitor cells in rat peripheral blood was assayed using flow cytometry. Expression of SDF-1α was evaluated by real-time polymerase chain reaction. RESULTS AND CONCLUSION: The healing area of wound surface was significantly increased in the honey group compared with saline group at 3 and 7 days (P < 0.05). The number of endothelial progenitor cells in peripheral blood was significantly increased at 3 days (P < 0.05). Real-time polymerase chain reaction demonstrated that SDF-1α m RNAexpression was significantly higher in the honey group compared with saline group. Results suggest that honey could accelerate mobilization of endothelial progenitor cells by production of SDF-1α, participate in neovascularization, and promote wound healing in diabetic rats Related Articles | Metrics

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Amniotic membrane loading epidermal stem cells accelerates wound healing in diabetic rats Zhong Qing-ling, Liu De-wu, Liu Fan-rong, Peng Yan, Yu Mei, Xiao Lu-liang 2010, 14 (32):  6010-6014.  doi: 10.3969/j.issn.1673-8225.2010.32.026 Abstract ( 83 )   PDF (556KB) ( 686 )   Save BACKGROUND: As the specific stem cells in skin tissues, epidermal stem cells (ESCs) which posses high proliferative potential and multiple-directional potential play a key role in repairing and rebuilding of the skin. Recent studies showed that the decreased amount and the reduced proliferation and differentiation capacity of ESCs may be one of the important mechanisms of difficult wound recovery in diabetes mellitus. OBJECTIVE: To investigate the effects of ESCs on wound healing of diabetic rats.  METHODS: ESCs of Sprague Dawley rats were isolated, cultured, identified and labeled with 5-bromo-2’-deoxyuridine (BrdU). The wound models of diabetic SD rats were established, and then divided into ESCs, HAM, blank control groups. Human amniotic membrane (HAM) loading labeled BrdU ESCs were implanted to ESCs group. HAM was implanted to HAM group, but blank control group was not intervened. General situation of wound healing was observed, and the healing rate of wound was calculated. Hematoxylin and eosin staining and immunohistochemical staining (SP method) were used to detect BrdU and expression of proliferating cell nuclear antigen in healing tissues. Mean value of integral absorbance of positive cells was measured with image analysis software. RESULTS AND CONCLUSION: At 7 days after treatment, the wounds of ESCs group reduced significantly. At 14 days after treatment, wounds of ESCs group were almost completely healed. Compared with HAM and blank control groups, the wound healing rate of ESCs group was significantly higher (P < 0.01). BrdU-positive cells in the wounds and newborn epidermis of ESCs group were visible, while wound tissue of HAM and blank control groups had no BrdU-positive cells. Proliferating cell nuclear antigen-positive cells in wound tissue of each group could be seen, but mean value of integral absorbance of positive cells showed significantly differences in ESCs group compared to HAM and blank control groups (P < 0.01). The results have confirmed that ESCs have a direct correlation with epidermal migration of wound margin and wound epithelialization in diabetic rats, may contribute to healing of diabetic skin wound. Related Articles | Metrics

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Hematopoietic reconstruction in mice treated with lethal dose irradiation using Sca-1+ hematopoietic stem/progenitor cells transplantation  Zhou Yue, Yang Bin, Yao Xin, Wang Ya-ping 2010, 14 (32):  6015-6019.  doi: 10.3969/j.issn.1673-8225.2010.32.027 Abstract ( 167 )   PDF (331KB) ( 461 )   Save BACKGROUND: In recent years, hematopoietic stem cell transplantation has widely used in clinic. The effectiveness of HSC reconstruction of the hematopoiesis has became a hot topic in the field of HSCT. OBJECTIVE: To study hematopoietic reconstruction for hematopoietic failure mice with the transplantation of Sca-1+ hematopoietic stem/progenitor cells (Sca-1+HSC/HPC). METHODS: The female C57BL/6 recipient mice received a lethal dose irradiation from 60Coγ ray and were divided into two groups. The control group mice were injected with sterile phosphate buffered saline. The transplantation group mice were transplanted with Sca-1+HSC/HPC isolated by magnetic cell sorting from homologous series male mice. After transplantation, the survival time, the change of peripheral blood leucocyte, haematocrit and platelet, the weight of spleen, the number of colony forming unit-spleen (CFU-S) of recipient mice were observed. Polymerase chain reaction (PCR) was used to measure Y chromosome (Sry gene) to verify the cell source of hematopoietic reconstruction in receipient mice. RESULTS AND CONCLUSION: The 30-day survival rate, the number of leucocyte, haematocrit and platelet, the weight of spleen and the number of CFU-S were significantly higher in transplantation group compared with control group (P < 0.05). Y chromosome PCR results indicated that reconstructed hematopoietic cells of female recipient mice were derived from male donor. These suggested that Sca-1+HSC/HPC transplantation can reconstruct hematopoietic function of hematopoietic failure mice. Related Articles | Metrics

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Application of vascular tissue engineered seed cells to regeneration medicine Li Chun-min, Wang Zhong-gao 2010, 14 (32):  6022-6026.  doi: 10.3969/j.issn.1673-8225.2010.32.029 Abstract ( 76 )   PDF (354KB) ( 359 )   Save BACKGROUND: Small bore artificial vessels to substitute human body arteriole and vein have not obtained satisfactory outcomes. Thus, to develop a small bore artificial vessels with high long-term patency rate has been a key research program. OBJECTIVE: To review the latest progress of seed cells in vascular tissue engineering.  METHODS: A computer based search was conducted in Pubmed database for articles concerning vascular tissue engineered seed cells published from 1960 to 2009 with the key words of “vascular tissue engineering, seeding cells”. The literatures with simple design, poor reliability, non English or repetitive results were excluded. Finally, 35 articles were included for further use. RESULTS AND CONCLUSION: The endothelial cells and smooth muscle cells were commonly used in the vascular tissue engineering. Endothelial cells and smooth muscle cells simulate in vivo environment to keep their normal secretory function and phenotype. Bone marrow mesenchyme stem cells can be effectively isolated and amplified, and can differentiate into various vascular cells under the special conditions and have strong potential in regeneration medicine and biological tissue engineering. Related Articles | Metrics

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Umbilical cord blood stem cells: New seed cells for the research of construction of tissue engineered artificial heart Chen Gui-xiu, Feng Bin, Liu Tao 2010, 14 (32):  6027-6030.  doi: 10.3969/j.issn.1673-8225.2010.32.030 Abstract ( 121 )   PDF (336KB) ( 351 )   Save BACKGROUND: Umbilical cord blood stem cells contain a variety of stem/progenitor cells. Usage of these stem/progenitor cells for transplantation therapy and as seed cells in tissue engineering research is a current research focus. OBJECTIVE: To review the biological characteristics of various types of stem /progenitor cells contained in umbilical cord blood stem cells and related stem cell-based experimental application for treatment of heart diseases. METHODS: To search related literatures by computer retrieval in China Hospital Knowledge Database and Chongqing VIP information web by using key words as “umbilical cord blood stem cell, hematopoietic progenitor/ stem cells, mesenchymal stem cells, endothelial progenitor cells, endothelial cells, myocardial cells, tissue engineering, seed cells” in Chinese from January 1999 to October 2009. Further literatures searching was tried in PubMed database with search terms limited to “umbilical cord blood stem cell, hematopoietic progenitor cells/hematopoietic stem cells, mesenchymal stem cells, endothelial progenitor cells, endothelial cells, myocardial cells, tissue engineering, seed cells” in English from January 1999 to October 2009. In addition, manual retrieval of the monograph of Stem Cell Biology was also done. A total of 95 documents were retrieved. According to the inclusion criteria, 30 documents were finally selected. RESULTS AND CONCLUSION: Umbilical cord blood stem cells are a kind of adult multipotent stem cells, including hematopoietic progenitor cells/hematopoietic stem cells, mesenchymal stem cells, endothelial progenitor cells, unrestricted somatic stem cells and so on. Umbilical cord blood stem cells contain rich primitive stem cells, which have stronger differentiation and proliferation capacity, smaller rejection reaction and get more easily than that of bone marrow stem cells and peripheral blood stem cells. Umbilical cord blood stem cells can differentiate into many kinds of cells such as myocardial cells and vascular endothelial cells in vitro. Based on the unique biological characteristic and source superiority of umbilical cord blood stem cells, the outcome in treatment of coronary heart disease and ischemic cardiomyopathy had obtained. Umbilical cord blood stem cells may be a new source of seed cells for construction tissue engineered artificial heart, and show broad prospects for research of heart regeneration and tissue engineered artificial heart. Related Articles | Metrics

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Current research of differentiation from different sources-derived stem cells into neural cells Zhuge Dong, Liang Zhan-hua, Song Lin, Liu Jing 2010, 14 (32):  6031-6035.  doi: 10.3969/j.issn.1673-8225.2010.32.031 Abstract ( 100 )   PDF (386KB) ( 438 )   Save BACKGROUND: Refractory nervous system diseases, including degenerative diseases, stroke sequelae, brain and spinal cord injury, mainly present a large number of neuronal necrosis and loss of function. Adult mammalian central nervous system regeneration after injury is poor, almost impossible to generate new neurons, or to complete self-repair. The recovery of function in injured or degenerated brain tissue has not been able to solve. Stem cell research and application offered hope for patients with nervous system disease. OBJECTIVE: Studies on stem cells have become a hot point in recent years. We are in attempt to search the relevant literature of the differentiation from different sources-derived stem cells into neural cells, and to compare the ability of differentiation from different sources-derived stem cells into neural cells. METHODS: We searched for the related articles in PubMed published from January 1995 and December 2009 in recent five years. The key words were “neuronal differentiation, embryonic stem cells, bone marrow mesenchymal stem cells. adipose derived mesenchymal stem cells, peripheral blood stem cells, umbilical cord blood stem cells” in English. The articles were selected primarily and the attached literatures were looked through. The articles were related to differentiation from different sources-derived stem cells into neural cells. RESULTS AND CONCLUSION: Ideal seed cells should be minimally invasive, easy to access, easy-to-amplify in vitro, do not involve the immune rejection of tumorigenicity and ethical issues. These advantages have not yet found in the seed cells. The ideal seed cells derived not only to consider the legal issues and more importantly, directed differentiation of cells. Ideal cells should be selected according to the ability of stem cells during stem cell transplantation in the nervous system. There are great differences in research outcomes concerning the ability of differentiation of stem cells into neural cells, so ideal seed cells cannot be identified. Future research needs to study the differentiation of stem cells from different sources into neural cells. Related Articles | Metrics

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Research progress addressing muscle-derived stem cells injection for treatment of stress urinary incontinence Tu You-bing, Li Ai-bin 2010, 14 (32):  6036-6039.  doi: 10.3969/j.issn.1673-8225.2010.32.032 Abstract ( 122 )   PDF (330KB) ( 349 )   Save BACKGROUND: Injection therapy for stress urinary incontinence is a minimally invasive means of treatment, but because of previous vaccination materials restriction, there are many complications, with poor long-term effects. It is significant to search new injection materials. OBJECTIVE: To retrospectively analyze the research of stress urinary incontinence using muscle-derived stem cells. METHODS: The first author retrieved PubMed database, Springer database and China National Knowledge Infrastructure for articles concerning muscle-derived stem cells for treatment of stress urinary incontinence published from 2003 to 2010. The key words were “stress urinary incontinence, muscle-derived stem cell”. A total of 40 articles were retrieved primarily by computer, and 24 articles were finally included for review according to inclusion criteria. RESULTS AND CONCLUSION: Treatment method of stress urinary incontinence mainly included non-surgery therapy and surgery therapy. The surgery treatment exists many complications, even risk for overcorrect. However, the injection therapy, as a non-surgery therapy, was simple to operate, could be accepted by patients. Muscle-derived stem cells following injection have many advantages, such as long-term survival, not easy to broken down, not easy to move, low immunogenicity, and will have a bright prospect in the future. Related Articles | Metrics

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null Zhang Hui-feng, Zhao Zhi-gang, Bai Wei-xing 2010, 14 (32):  6040-6043.  doi: 10.3969/j.issn.1673-8225.2010.32.033 Abstract ( 109 )   PDF (273KB) ( 405 )   Save BACKGROUND: Intramuscular injection transplantation of autologous bone marrow stem cells for the treatment of limb ischemia has obtained good outcomes, which have been confirmed by animal test and clinic. Recently, small balloon dilatation is a common method to treat diabetic lower limb arteriosclerosis. However, stem cell transplantation and interventional therapy by catheter infusion of autologous bone marrow stem cells are commonly used to treat coronary artery disease, but seldom used for treating arterial occlusion of the lower limb. OBJECTIVE: To evaluate the clinical efficacy of transplantation of autologous bone marrow stem cells for the treatment of diabetic lower limb arterial occlusion. METHODS: From June 2007 to June 2009, a total of 51 patients with diabetic lower limb arterial occlusion (27 males and 24 females) were enrolled at the Department of Endocrinology, Geriatric Medicine Branch, Henan Provincial People’s Hospital, and randomly divided two groups. Patients in the experimental group underwent small balloon dilatation and autologous bone marrow stem cells transplantation. Patients in the control group received small balloon dilatation. Pain, cold feeling, intermittent claudication before and after transplantation were observed, as well as ankle brachial index changes and complications following transplantation were observed. RESULTS AND CONCLUSION: At 2 week posttransplantation, remission rate, improvement rate and inefficiency of lower-limb pain symptom, cold feeling and intermittent claudication were significantly greater in the experimental group compared with the control group (P < 0.01). There were significant differences in ankle-brachial index changes (P < 0.01). No complication or adverse effects were observed. Results suggest that transplantation of autologous bone marrow stem cells through balloon dilatation might be a simple, safe and effective method for diabetic lower limb arterial occlusion. References | Related Articles | Metrics

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Transplantation of endothelial progenitor cells transfected with human telomerase reverse transcriptase gene for treatment of rat myocardial infarction  Li Juan, Zhang Shu-lin 2010, 14 (32):  6044-6047.  doi: 10.3969/j.issn.1673-8225.2010.32.034 Abstract ( 81 )   PDF (306KB) ( 257 )   Save BACKGROUND: Many animal experiments showed that endothelial progenitor cells (EPCs) could ameliorate myocardial ischemia and increase collateral circulation after myocardial infarction. However, EPCs are less in the peripheral blood and the contents of the amplified cells are also very limited. OBJECTIVE: To observe proliferation and differentiation of EPCs transfected with telomerase reverse transcriptase gene, and effects of EPC transplantation on neovascularization and cardiac function in the region of myocardial infarction. METHODS: A computer-based search was performed in PubMed and Chinese Journal Full-text Database for relevant articles published from January 1993 to April 2010. The key words were “endothelial progenitor cells, human telomerase reverse transcriptase, myocardial infarction, seed cells”. To focus on thirty documents, we discussed the effect of EPCs transfected with telomerase reverse transcriptase gene in treatment of myocardial infarction.  RESULTS: Human telomerase reverse transcriptase gene could be successfully transfected into EPCs, and EPCs transfected with human telomerase reverse transcriptase gene could enhance the abilities of proliferation, prolong life, and improve the abilities of neovascularization and endomembrane repair in order to treat myocardial infarction. There is no report concerning EPCs transfected with human telomerase reverse transcriptase for treatment of myocardial infarction. CONCLUSION: The EPCs modified by human telomerase reverse transcriptase gene could enhance the abilities of proliferation, and increase activity of EPCs, improve neovascularization and improve cardiac function after myocardial infarction. Related Articles | Metrics

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Targeted therapy of surface molecules, regulatory mechanisms and microenvironment of leukemia stem cells  Zhou Mei, Ouyang Chenxi 2010, 14 (32):  6048-6051.  doi: 10.3969/j.issn.1673-8225.2010.32.035 Abstract ( 121 )   PDF (327KB) ( 350 )   Save BACKGROUND: With the proposition and deep research of leukemia stem cells, it is possible to find out new ways to treat leukemia more effectively, which are targeting leukemia stem cells. OBJECTIVE: To review the research concerning leukemia stem cells. METHODS: A computer-based search was undertaken in Vip database for articles published from January 1989 to January 2010 with the key words of “leukemia stem cells” in Chinese. Simultaneously, Foreign Medical Journal Service was searched for the articles about leukemia stem cells published between January 1995 and January 2010, with the key words of “leukemia stem cells” in English. A total of 154 articles were collected. RESULTS AND CONCLUSION: With the proposal of leukemia stem cells concept and deep research, the targeted therapy for leukemia stem cells became possible. Through the isolation, purification and identification of leukemia stem cells, experts tried to develop new ways to treat leukemia, which are targeting cellular surface molecules, regulatory mechanisms and microenvironment of leukemia stem cells. Primary results proved that these methods are feasible. However, some confounding factors should be excluded. Animal models are difficult to completely simulate in vivo environment. Related Articles | Metrics

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Human leukocyte antigen-mismatched hematopoietic stem cell transplantation in 15 patients with myelodysplastic syndromes  Zhang Yuan, Chen Hui-ren, He Xue-peng, Lou Jin-xing, Liu Xiao-dong, Guo Zhi, Yang Kai, Chen Peng 2010, 14 (32):  6052-6055.  doi: 10.3969/j.issn.1673-8225.2010.32.036 Abstract ( 109 )   PDF (334KB) ( 376 )   Save BACKGROUND: Myelodysplastic syndromes (MDS), a series of heterogenicity myeloblastic tumor, have transformed to leucemia easily. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only possible treatment for curing this disease. OBJECTIVE: To observe the therapeutic effect and complication of human leucocyte antigen (HLA)-mismatched HSCT for MDS, and to evaluate the outcome and safety of this treatment. METHODS: A total of 15 patients with MDS were treated with HLA-mismatched HSCT. The conditioning regimen contained arabinosylcytosin, busulfan, fludarabine and methyl chlorethyl-cyclohexyl-nitrosourea. Antithymocyte globulin, ciclosporin A, mycophenolate mofetil and methotrexate were used for preventing graft versus host disease (GVHD). RESULTS AND CONCLUSION: Among 15 patients, 14 received hematopoietic recovery after HSCT. The median time of granulocyte recovery ≥ 0.5×109/L and platelets ≥ 20×109/L were day 16 and day 20 respectively. One patient accepted the second time HSCT after failing of the first treatment, and received hematopoietic recovery on day 14 following transplantation. A total of 14 cases survived for a median of 14 (1-36) months, and one died from pulmonary infection, accumulated survival rate was 93.3%. One patient relapsed in 12 months after HSCT, and received complete remission after accepted donor lymphocyte infusion and chemotherapy. Eight patients occured acute GVHD (aGVHD) after HSCT, resulting in the incidence rate of 57.1%. Two of them were controlled after treatment with immunodepressant, and the remaining six developed chronic GVHD (cGVHD). Results suggested that HSCT is an effective treatment for MDS patients, and a majority of patients can survive for a long time. Related Articles | Metrics

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Autologous hematopoietic stem cell transplantation for the treatment of type 1 diabetes mellitus combined with hypothyroidism in two cases Jing Hua, Zhang Jin-yuan, Lü Xu-jing, Shen Xu-dong 2010, 14 (32):  6056-6059.  doi: 10.3969/j.issn.1673-8225.2010.32.037 Abstract ( 91 )   PDF (271KB) ( 318 )   Save BACKGROUND: Previous studies have confirmed that the outcome of hematopoietic stem cell transplantation for treatment of type 1 diabetes mellitus is identified. OBJECTIVE: To observe the safety and efficacy of autologous hematopoietic stem cells transplantation in treatment of type 1 diabetes mellitus combined with hypothyroidism. METHODS: A total of two cases of type 1 diabetes mellitus and hypothyroidism were subjected to immune clearance and hematopoietic stem cell transplantation. The hematopoietic stem cell mobilization, collection, immune suppression and hematopoietic stem cell reinfusion all achieved satisfactory results. Insulin, levothyroxine sodium content, serum C peptide mass concentration, glycosylated hemoglobin level and thyroid gland function differences were compared prior to and following treatment. RESULTS AND CONCLUSION: C peptide mass concentration recovered to normal in 1 case following treatment. Insulin was withdrawn. Dose of thyroxine tablets was reduced and thyroid function resulted in the normal range. In another case, insulin dose reduced by 64%. Withdraw of thyroxine tablets resulted in normal thyroid function. These indicated that it is safe to treat type 1 diabetes mellitus combined with hypothyroidism, resulting in obvious short-term outcomes. Related Articles | Metrics

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Feasibility of stem cells from umbilical cord blood as seed cells for tooth regeneration Ji Bao-hui, Chen Jiao, Wang Hang 2010, 14 (32):  6060-6063.  doi: 10.3969/j.issn.1673-8225.2010.32.038 Abstract ( 73 )   PDF (236KB) ( 735 )   Save BACKGROUND: With the development of tissue engineering, more attention has been paid to tooth regeneration. However, the resource of best seed cells is still uncertain. Therefore, alternative sources should be attached to intensive investigation. OBJECTIVE: To review the feasibility of stem cells from umbilical cord blood as seed cells for tooth regeneration. METHODS: A PubMed search was performed for articles published between January 1998 and January 2009. Key words were “tooth regeneration, seed cells, tissue engineering, umbilical cord blood, mesenchymal stem cells”. Only studies written in English were included. Simultaneously, databases of China National Knowledge Infrastructure, Wanfang, and VIP published between January 1998 and January 2009 were also retrieved, using the key words of “tooth regeneration, seed cells, tissue engineering, umbilical cord blood, mesenchymal stem cells”. Only studies written in Chinese were included. Totally, 40 literatures were included. RESULTS AND CONCLUSION: Seed cells, such as odontoblasts from dental germ, stem cells from dental pulp and deciduous teeth, and ectomesenchymal cells from the firstbranchial arch showed odontogenic differentiation potential in basic research. However, these cells are not practical to harvest in clinic. Although bone marrow mesenchymal stem cells have odontogenic capacity, their differentiation abilities significantly decrease with the increasing age of the donors. Stem cells from umbilical cord blood have many clinical advantages over bone marrow mesenchymal stem cells, and exhibited typical mesenchymal stem cells characteristics. Thus, we propose the hypotheses that stem cells from umbilical cord blood could be induced into odontogenic lineage and might be used as suitable seed cells for tooth regeneration to replace the lost tooth. Related Articles | Metrics

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Transdifferentiation of mouse pancreatic ductal epithelial cells into islet-like cells Zhao Yan-yan, Yu Qin, Li Zhi-zhen, Qin Gui-jun 2010, 14 (32):  6064-6067.  doi: 10.3969/j.issn.1673-8225.2010.32.039 Abstract ( 145 )   PDF (261KB) ( 422 )   Save BACKGROUND: Islet transplantation is an effective method for the treatment of type 1 diabetes mellitus and parts of type 2 diabetes mellitus. However, its application is hindered by insufficient sources and immunologic rejection. Though transdifferentiation of pancreatic stem cells is at the starting step, it is thought to be the hopeful source for islet cell transplantation. OBJECTIVE: To look for a suitable cells-transplantation source for the treatment of diabetes mellitus. METHODS: The pancreatic ductal epithelial cells were separated from Kunming mice and cultured in DMEM/F12 medium supplemented with keratinocyte growth factor, hepatocyte growth factor and nicotinamide, etc. Samples were taken at different time points for light microscopy and electron microscope. The changes of CK-19 and PDX-1 were detected by immunocytochemistry at 1 and 16 days. The expressions of insulin and glucagon gene were detected by RT-PCR at 1 and 16 days. The physiologic function of these islet-like clusters was determined by dithizone staining and glucose stimulation at 21 days.  RESULTS AND CONCLUSION: A large number of epitheliod cells were CK-19 immunoreactive positive and few of them were PDX-1 positive at 1 day after isolation, then CK-19 positive cells proliferated quickly and formed substantial plaques of epithelial cells in cobblestone pattern. At 16 days later, these cells begin to form islet-like clusters gradually, while most of them were PDX-1 immunoreactive positive. The analysis of mRNA by RT-PCR showed very low levels of insulin and glucagon mRNA in the starting materials but increase was found as the process of transdifferentiation. At 21 day differentiated islet-like clusters were stained red by dithizone. In those samples exposed to a stimulatory 15 mmol/L glucose, there was a 1.6-fold increase in insulin compared with to 5.6 mmol/L glucose (P < 0.05). Pancreatic ductal cells of adult Kunming mice could proliferate quickly and have the potency of transdifferentiation into islet-like clusters when cultured in vitro under appropriate conditions. Related Articles | Metrics

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Feridex labeling of bone marrow stromal cells of crab-eating macaque Chen Xia, Yang Zhi-jun, Luo Yong-chun, Cai Ying-qian, Du Mou-xuan, Zou Yu-xi 2010, 14 (32):  6068-6072.  doi: 10.3969/j.issn.1673-8225.2010.32.040 Abstract ( 101 )   PDF (421KB) ( 746 )   Save BACKGROUND: Studies regarding Feridex in vitro cell labeling are mainly in rodents, while little information is known on primate crab-eating macaque. OBJECTIVE: To explore the feasibility of protocols using Feridex and transfection agents for in vitro magnetic labeling of bone marrow stromal cells (BMSCs) in crab-eating macaque. METHODS: Under the sterile condition, the crab-eating macaque BMSCs were obtained by means of density gradient centrifugation following a bone puncture. Feridex-poly-l-lysine complexes were used to magnetically label BMSCs. The efficiency and cellular viability of Feridex-poly-l-lysine labeled BMSCs were evaluated by Prussian blue staining, electron microscopy, and trypan blue dye exclusion test. The proliferation and differentiation ability of Feridex-poly-l-lysine labeling BMSCs were also investigated by inverted phase contrast microscope and immunocytochemistry. RESULTS AND CONCLUSION: BMSCs could be effectively labeled by Feridex and labeling efficiency was around 99%. Tiny blue stained fine particles and numerous vesicles coated with the electron-dense magnetic iron particles could be found in the cytoplasm of Feridex-poly-l-lysine labeled BMSCs under optical microscopy and transmission electron microscopy respectively. Cell viability, proliferation and differentiation ability of labeled BMSCs were not affected by Feridex-poly-l-lysine labeling. Results indicated that Feridex might be used to label BMSCs of crab-eating macaque. Related Articles | Metrics

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Haploidentical hematopoietic stem cells transplantation for the treatment of medulloblastoma Hu Hai-yan, Guo Hong-bo, Wu Bing-yi, Deng Lan, Song Chao-yang, Guo Kun-yuan 2010, 14 (32):  6073-6076.  doi: 10.3969/j.issn.1673-8225.2010.32.041 Abstract ( 94 )   PDF (245KB) ( 322 )   Save BACKGROUND: Some reports demonstrated that, autoallergic or hematopoietic stem cells transplantation combined with chemotherapy received good outcomes in treating medulloblastoma, which can prolong survival time of patients. However, whether haploidentical hematopoietic stem cells transplantation can treat medulloblastoma remains poorly understood. OBJECTIVE: To firstly report a patient receiving haploidentical hematopoietic stem cells transplantation for treating medulloblastoma. METHODS: A terminal cancer patient with bone matastases successively received six lymphocyte transfusions from an unrelated donor combined with chemotherapy and three haploidentical hematopoietic stem cell transplantations. RESULTS AND CONCLUSION: The patient presented erythra, accompanied by fever, diarrhea and yellow brown liquid stools, which was considered as graft versus host disease, and treated by urbason, gammaglobulin, CellCept, Prograf, Basiliximab (anti-CD25 antibody), Infliximab (anti-tumor necrosis factor α antibody), effective antibacterial and supportive treatments. After that, the erythra and diarrhea were remised. But the patient died from cerebral hemorrhage. Allogeneic lymphocyte transfusion can kill or damage tumor cells, improve life quality, but the outcome is restrained for patient with a high tumor burden. Immunosuppressant, such as anti-CD25 antibody and anti-tumor necrosis factor α antibody should be timely used in consideration of allogeneic hematopoietic stem cell transplantation. Related Articles | Metrics