Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 5944-5948.doi: 10.3969/j.issn.1673-8225.2010.32.012

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Isolation of human placenta derived mesenchymal stem cells by enzymatic digestion versus global perfusion

Li Fang 1,2 , Miao Zong-ning3, Xu Yun-yun4, Zhang Xue-guang 1,2   

  1. 1 Department of Human Anatomy, Histology and Embryology, College of Basic Medicine and Biological Science, Soochow University, Suzhou  215007, Jiangsu Province, China; 2 Jiangsu Stem Cell Key Laboratory, Suzhou  215007, Jiangsu Province, China; 3 Burn Institute, Wuxi Third People’s Hospital, Wuxi  214041, Jiangsu Province, China; 4 Institute of Pediatrics, Children Hospital Affiliated to Soochow University, Suzhou  215007, Jiangsu Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Zhang Xue-guang, Professor, Doctoral supervisor, Department of Human Anatomy, Histology and Embryology, College of Basic Medicine and Biological Science, Soochow University, Suzhou 215007, Jiangsu Province, China; Jiangsu Stem Cell Key Laboratory, Suzhou 215007, Jiangsu Province, China xueguangzh@yahoo.com.cn
  • About author:Li Fang☆, Studying for doctorate, Lecturer, Department of Human Anatomy, Histology and Embryology, College of Basic Medicine and Biological Science, Soochow University, Suzhou 215007, Jiangsu Province, China; Jiangsu Stem Cell Key Laboratory, Suzhou 215007, Jiangsu Province, China mercuryfish@163.com

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Abstract:

BACKGROUND: The method of isolation and purification of human placenta derived mesenchymal stem cells (PMSCs) has certain blindness due to the interference of other cells within the placenta.
OBJECTIVE: To investigate the efficiency of two methods to isolate PMSCs such as enzyme digestion and global perfusion, to find an efficient method and to establish a stable in vitro culture system of PMSCs.
METHODS: The normal term placenta was obtained to isolate PMSCs by different methods. Ⅳ collagenase digestion was used in the enzymatic digestion group, and global perfusion was utilized in the global perfusion group. The growth characteristics of cells, cell surface marker, as well as the multiple differentiation potential of the cultured cell obtain by these two methods were compared following conventional culture.
RESULTS AND CONCLUSION: There was no significant difference of the cell growth, multiple differentiation potential between the cultured cell obtain by these two method mentioned above, but the CD90 was obviously lowly expressed on the cultured cells obtained by enzymatic digestion compared with global perfusion (P < 0.05), which shows that global perfusion is a more efficient way to get higher purity of PMSCs compared with enzymatic digestion.

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