Silicate-Cu/Mg bioactive ceramics promote the osteogenesis of osteoblasts

doi:10.3969/j.issn.2095-4344.2327

Abstract

Abstract:

BACKGROUND: The use of silicate bioceramics as a tissue-engineered bone scaffold has poor ability to promote osteogenesis. Studies have shown that copper, magnesium, and other essential trace elements have obvious effects on the induction and stimulation of osteoblasts and hemangioblasts.

OBJECTIVE: To investigate the effects of silicate bioactive ceramics with Cu and Mg on osteoblast proliferation and osteogenesis.

METHODS: Cu-silicate bioceramics, Mg-silicate bioceramics, and Cu-Mg-silicate bioactive ceramics were prepared by the sol-gel method (molar ratio of both Cu and Mg in ceramics was 5%). Three experimental groups were CS-5Cu, CS-5Mg, CS-5Cu/5Mg groups. The silicate bioactive ceramics served as the control group (denoted as CS). X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy were used to characterize the samples. The surface crystallization of bioceramics was detected. Osteoblasts were co-cultured with four groups of ceramics for 24 hours. Osteoblast proliferation index, alkaline phosphatase secretion, osteopontin and osteocalcin gene expression, vinculin and actin protein expression were determined.

RESULTS AND CONCLUSION: (1) The crystallization ability of different silicate bioceramic samples followed the order of CS-5Cu>CS>CS-5Cu/5Mg>CS-5Mg. (2) Osteoblast proliferation index followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (3) Alkaline phosphatase secretion was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (4) Osteopontin and osteocalcin gene expression followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (5) Vinculin and actin protein expression was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (6) These results suggest that Cu- or Mg-silicate, in particular Cu-Mg-silicate bioactive ceramics can promote the proliferation of osteoblasts and the expression of osteogenesis-related genes as well as cell adhesion and spreading.

Key words: silicate bioactive ceramics, osteoblasts, osteogenic properties, biological activity, osteoblast gene expression, cytoskeletal morphology

CLC Number:

Zhou Hangyu, Zeng Fuhai, Xia Delin. Silicate-Cu/Mg bioactive ceramics promote the osteogenesis of osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(28): 4511-4517.

Figures/Tables 10

References

[1] WENG L, BODA SK, TEUSINK MJ, et al. Binary Doping of Strontium and Copper Enhancing Osteogenesis and Angiogenesis of Bioactive Glass Nanofibers while Suppressing Osteoclast Activity.ACS Appl Mater Interfaces. 2017;9(29): 24484-24496.

[2] ROGERS GF, GREENE AK. Autogenous bone graft: basic science and clinical implications.J Craniofac Surg. 2012;23(1): 323-327.

[3] KIERNAN CH, WOLVIUS EB, BRAMAPAJ, et al. The Immune Response to Allogeneic Differentiated Mesenchymal Stem Cells in the Context of Bone Tissue Engineering.Tissue Eng Part B Rev. 2018;24(1):75-83.

[4] KIM HD, AMIRTHALINGAM S, KIM SL, et al. Biomimetic Materials and Fabrication Approaches for Bone Tissue Engineering.Adv Healthc Mater. 2017;6(23). doi:10.1002/adhm.201700612.

[5] NOORI A, ASHRAFI SJ, VAEZ-GHAEMI R, et al. A review of fibrin and fibrin composites for bone tissue engineering.Int J Nanomedicine.2017;12:4937-4961.

[6] BAINO F, POTESTIO I, VITALE-BROVARONE C, et al. Production and Physicochemical Characterization of Cu-Doped Silicate Bioceramic Scaffolds.Materials(Basel). 2018;11(9):1524.

[7] LEU A, LEACH JK. Proangiogenic potential of a collagen/ bioactive glass substrate.Pharm Res. 2008;25(5):1222-1229.

[8] LEU A, STIEGER SM, DAYTON P, et al. Angiogenic response to bioactive glass promotes bone healing in an irradiated calvarial defect.Tissue Eng Part A.2009;15(4):877-885.

[9] DAY RM. Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro.Tissue Eng.2005;11(5-6): 768-777.

[10] SHI F, LIU Y, ZHI W, et al. The synergistic effect of micro/nano-structured and Cu2+-doped hydroxyapatite particles to promote osteoblast viability and antibacterial activity. Biomed Mater.2017;6;12(3):035006.

[11] REN L, WONG HM, YAN CH, et al. Osteogenic ability of Cu-bearing stainless steel.J Biomed Mater Res B Appl Biomater. 2015;103(7):1433-1444.

[12] HU K, OLSEN BR. Osteoblast-derived VEGF regulates osteoblast differentiation and bone formation during bone repair.J Clin Invest. 2016;126(2):509-526.

[13] PRADO FERRAZ E, PEREIRA FREITAS G, CAMURI CROVACE M, et al. Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering. Biomed Mater.2017;12(4): 045018.

[14] THOMAS A, ERA J. Preparation and characterization of gelatin-bioactive glass ceramic scaffolds for bone tissue engineering. J Biomater Sci Polym Ed.2019;30(7):561-579.

[15] NIKPOUR P, SALIMI-KENARI H, FAHIMIPOUR F, et al. Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bonetissue engineering. Carbohydr Polym.2018;15;190:281-294.

[16] FENG Y, LI Q, WU D, et al A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis.Biomaterials.2017;134:128-142.

[17] VANHATUPA S, MIETTINEN S, PENA P, et al. Diopside-tricalcium phosphate bioactive ceramics for osteogenic differentiation of human adipose stem cells.J Biomed Mater Res B Appl Biomater. 2019;108(3):819-833.

[18] DENG C, CHANG J, WU CT, et al. Bioactive scaffolds for osteochondral regeneration.J Orthop Translat.2018;26;17:15-25.

[19] MANTOVANI A, LOCATI M. Macrophage Metabolism Shapes Angiogenesis in Tumors.Cell Metab. 2016;24(6):887-888.

[20] RAU JV, CURCIO M, RAUCCI MG, et al. Cu-Releasing Bioactive Glass Coatings and Their in Vitro Properties.ACS Appl Mater Interfaces.2019;13;11(6):5812-5820.

[21] HELLBERG C, OSTMAN A, HELDIN CH, et al. PDGF and vessel maturation.Recent Results Cancer Res. 2010;180:103-114.

[22] MILKOVIC L, HOPPE A, RAUCCI MG, et al. Effects of Cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cells in vitro.J Biomed Mater Res A. 2014;102(10):3556-3561.

[23] TAMPIERI A, RUFFINI A, BALLARDINI A, et al. Heterogeneous chemistry in the 3-D state: an original approach to generate bioactive, mechanically-competent bone scaffolds.Biomater Sci. 2018;18;7(1):307-321.

[24] VARADINOVA TL, ZLATEVA KT, DYULGEROVAEI, et al. Cell response to herpes simplex virus type 1 infection mediated by biphasic calcium-phosphateceramics: in vitro approach.J Biomed Mater Res.2001;57(2):232-237.

[25] ROMAN J, SALINAS AJ, VALLET-REGIM, et al. Role of acid attack in the in vitro bioactivity of a glass-ceramic of the 3CaO. P2O5-CaO.SiO2-CaO.MgO.2SiO2 system. Biomaterials.2001; 22(14):2013-2019.

[26] KOKUBO T, KUSHITANI H, SAKKA S, et al. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W.J Biomed Mater Res.1990;24(6):721-734.

[27] LI H, XUE K, KONG N, et al. Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells. Biomaterials. 2014;35(12):3803-3818.

[28] CAVEDA L, MARTIN-PADURA I, NAVARRO P, et al. Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin).J Clin Invest.1996;98(4):886-893.

[29] CHING HS, LUDDIN N, RAHMAN IA, et al. Expression of Odontogenic and Osteogenic Markers in DPSCs and SHED: A Review.Curr Stem Cell Res Ther.2017;12(1):71-79.

[30] MA J, WANG Z, ZHAO J. Resveratrol Attenuates Lipopolysaccharides (LPS)-Induced Inhibition of Osteoblast Differentiation in MC3T3-E1 Cells.Med Sci Monit. 2018;24: 2045-2052.

[31] YIN C, ZHANG Y, CAI Q, et al. Effects of the micro-nano surface topography of titanium alloy on the biological responses ofosteoblast.J Biomed Mater Res A.2017;105(3):757-769.

[32] PONTE AL, MARAIS E, GALLAY N, et al. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells.2007;25(7):1737-1745.

[33] STRATMAN AN, SCHWINDT AE, MALOTTE KM, et al. Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization.Blood.2010;116(22):4720-4730.