Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 6010-6014.doi: 10.3969/j.issn.1673-8225.2010.32.026

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Amniotic membrane loading epidermal stem cells accelerates wound healing in diabetic rats

Zhong Qing-ling 1,2, Liu De-wu2, Liu Fan-rong1, Peng Yan2, Yu Mei1, Xiao Lu-liang1   

  1. 1 Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2 Institute of Burn Surgery, First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Liu De-wu, Doctor, Professor, Chief physician, Doctoral supervisor, Institute of Burn Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China dewuliu@126.com
  • About author:Zhong Qing-ling☆, Studying for doctorate, Associate professor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China; Institute of Burn Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China Qingling5218@163. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30560058*; the Science and Technology Project of Education Department in Jiangxi Province, No. GJJ09115*; the Science and Technology Project of Health Department in Jiangxi Province, No. 20092021*

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Abstract:

BACKGROUND: As the specific stem cells in skin tissues, epidermal stem cells (ESCs) which posses high proliferative potential and multiple-directional potential play a key role in repairing and rebuilding of the skin. Recent studies showed that the decreased amount and the reduced proliferation and differentiation capacity of ESCs may be one of the important mechanisms of difficult wound recovery in diabetes mellitus.
OBJECTIVE: To investigate the effects of ESCs on wound healing of diabetic rats. 
METHODS: ESCs of Sprague Dawley rats were isolated, cultured, identified and labeled with 5-bromo-2’-deoxyuridine (BrdU). The wound models of diabetic SD rats were established, and then divided into ESCs, HAM, blank control groups. Human amniotic membrane (HAM) loading labeled BrdU ESCs were implanted to ESCs group. HAM was implanted to HAM group, but blank control group was not intervened. General situation of wound healing was observed, and the healing rate of wound was calculated. Hematoxylin and eosin staining and immunohistochemical staining (SP method) were used to detect BrdU and expression of proliferating cell nuclear antigen in healing tissues. Mean value of integral absorbance of positive cells was measured with image analysis software.
RESULTS AND CONCLUSION: At 7 days after treatment, the wounds of ESCs group reduced significantly. At 14 days after treatment, wounds of ESCs group were almost completely healed. Compared with HAM and blank control groups, the wound healing rate of ESCs group was significantly higher (P < 0.01). BrdU-positive cells in the wounds and newborn epidermis of ESCs group were visible, while wound tissue of HAM and blank control groups had no BrdU-positive cells. Proliferating cell nuclear antigen-positive cells in wound tissue of each group could be seen, but mean value of integral absorbance of positive cells showed significantly differences in ESCs group compared to HAM and blank control groups (P < 0.01). The results have confirmed that ESCs have a direct correlation with epidermal migration of wound margin and wound epithelialization in diabetic rats, may contribute to healing of diabetic skin wound.

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