Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 6006-6009.doi: 10.3969/j.issn.1673-8225.2010.32.025

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Honey for treatment of diabetic wounds and mobilization of endothelial precursor cells

Li Lan1, Lu Zu-qian2, Li Xiang2, Ji Qiu-he1, Xu Zhang-rong2, Cao Ming-jun2   

  1. 1 Department of Endocrinology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shanxi Province, China; 2 Department of Endocrinology, Military Diabetes Diagnosis and Treatment Center, the 306 Hospital of Chinese PLA, Beijing  100101, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Xu Zhang-rong, Master, Chief physician, Department of Endocrinology, Military Diabetes Diagnosis and Treatment Center, the 306 Hospital of Chinese PLA, Beijing 100101, China xzr1021@vip.sina.com
  • About author:Li Lan★, Master, Physician, Department of Endocrinology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China lilan309@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30850013*

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Abstract:

BACKGROUND: New vessel is a basic process of wound healing, and bone marrow-derived endothelial progenitor cells (BM-EPCs) play a key role in this condition. It can contribute to form new vessels in ulcer area that BM-EPCs mobilize from bone marrow to peripheral blood circulation and migrate to ulcer wound. Stromal cell-derived factor-1α (SDF-1α) is on e key molecule, which regulates this migration of the precursor cells of new vessels to damaged tissue.
OBJECTIVE: To investigate mobilization of endothelial progenitor cells following honey treatment and the effects of honey on SDF-1α expression.
METHODS: A round full-thickness skin wound of 1.8 cm diameter was created on the backs of streptozotocin (STZ) induced diabetic rats. The rats were randomized into two groups: saline group and honey group. The wound surface was observed. The number of endothelial progenitor cells in rat peripheral blood was assayed using flow cytometry. Expression of SDF-1α was evaluated by real-time polymerase chain reaction.
RESULTS AND CONCLUSION: The healing area of wound surface was significantly increased in the honey group compared with saline group at 3 and 7 days (P < 0.05). The number of endothelial progenitor cells in peripheral blood was significantly increased at 3 days (P < 0.05). Real-time polymerase chain reaction demonstrated that SDF-1α m RNAexpression was significantly higher in the honey group compared with saline group. Results suggest that honey could accelerate mobilization of endothelial progenitor cells by production of SDF-1α, participate in neovascularization, and promote wound healing in diabetic rats

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