Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (17): 3143-3147.doi: 10.3969/j.issn.1673-8225.201.17.026

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Inducing a femoral head necrosis model by microwave heating: Screening of optimal temperature and time  

Peng Wu-xun1, Wang Lei2, Deng Jin1, Geng Cheng-kui3, Li Yan-lin3, Gong Yue-kun3, Li Shi-he3   

  1. 1 Department of Emergency and Trauma Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China; 2 Institute of Occupational Medicine, Guiyang Center for Disease Control and Prevention, Guiyang  550004, Guizhou Province, China; 3 Department of Orthopaedics, First Affiliated Hospital of Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Online:2010-04-23 Published:2010-04-23
  • Contact: Wang Lei, Doctor, Attending physician, Institute of Occupational Medicine, Guiyang Center for Disease Control and Prevention, Guiyang 550004, Guizhou Province, China misswanglei@163.com
  • About author:Peng Wu-xun☆, Doctor, Associate professor, Master’s supervisor, Department of Emergency and Trauma Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China misswanglei@163.com
  • Supported by:

     the Program of Health Department of Guizhou Province, No. gzwkj2008-1-010*

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Abstract:

BACKGROUND: The existed femoral head necrosis (FHN) model can not reflex the clinical necrosis of femoral head correctly. Microwave heating provides a new approach for model preparation, but the concrete standard remains uncertain.
OBJECTIVE: To study the optimal temperature and time in preparing a FHN model induced by microwave heating.
METHODS: Totally 48 rabbits were randomly divided into 4 groups on the basis of the microwave temperature (50, 55, 60℃) and heating time (10, 20 minutes). The microwave antenna was inserted into the rabbit femoral head. The rabbits were sacrificed immediately and at 1, 2, 4, 8 and 12 weeks after operation. A series of examinations were performed including gross observation, X-ray, histology and MRI to observe the femoral head necrosis and repair status.
RESULTS AND CONCLUSION: Marrow partially solidified in the group (50 ℃, 10 minutes) at 1 week, and the osteonecrosis returned to normal at 8 weeks after operation. In the group (55 ℃, 10 minutes), marrow was completely coagulated at 1 week and low signal on T1 weighted images and increased signal on T2 images were identified at 2 weeks. Osteonecrosis and repair occurred at the same time at 4 weeks. At 12 weeks, the osteonecrosis continued and the repair stopped, and the femoral heads started to collapse. All femoral heads collapsed at 8 weeks in group (50 ℃, 20 minutes) and group (60 ℃, 10 minutes). Accordingly, microwave heating is a good method in developing FHN model. 55 ℃ and 10 minutes are the optimal temperature and time for the development of FHN model of rabbits induced by microwave heating.

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