Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (12): 2145-2148.doi: 10.3969/j.issn.1673-8225.2010.12.014

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Effect of nickel-titanium alloy surface oxidation on L-929-related apoptotic gene bax/bcl-2 mRNA expression

Feng Cui-juan1, Wang Qiang2, Zhang Xue1, Zhang Yang1, Qin Ke1, Lu Li3　   

  1. 1 Department of Orthodontics, 2 Department of Oral Materials, 3 Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Lu Li, Professor, Doctoral supervisor, Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China luli@mail.cmu.edu.cn
  • About author:Feng Cui-juan☆, Studying for doctorate, Lecturer, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China xzf7090@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 20072106*, 20082088*

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Abstract:

BACKGROUND: Nickel-titanium memory alloy released toxic Ni 2+ following corrosion, which could seriously influence human body. Inhibitory coating of TiN, TiO2, and HA was made at alloy surface, respectively, while the coating might effectively inhibit release of Ni 2+ and improve the biocompatibility. 
OBJECTIVE: To evaluate the effect of nickel-titanium memory alloy following surface oxidation on biocompatibility.
METHODS: NiTi-SMA was treated with surface oxidation. The sample was then rinsed with 10% HF + 40 % HNO3 + 50% H2O, ultrasonic-cleared in acetone, dried, oxygenized in H2O2, rinsed, dried, sterilized, and lixiviated. L-929 cells were cultured in vitro and assigned into negative control (culture medium), surface oxidation (surface oxidation + NiTi-SMA leaching liquor), and non-treatment groups (NiTi-SMA leaching liquor alone). Apoptotic gene bax/bcl-2 mRNA expressions were measured by PT-PCR.
RESULTS AND CONCLUSION: bax/bcl-2 mRNA expression in the negative control group was significantly higher than surface oxidation and non-treatment groups, but bax/bcl-2 in the negative control group was significantly lower than surface oxidation and non-treatment groups (P < 0.01). There was no significant difference in bax/bcl-2 mRNA and bax/bcl-2 between surface oxidation and non-treatment groups (P > 0.05). This suggested that the nickel ion releasing from nickel-titanium memory alloy leaching liquor could result in the variation of bax/bcl-2 mRNA expression in L-929, which may be used as an important index to evaluate the biocompatibility of biomaterials.

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