Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (12): 2141-2144.doi: 10.3969/j.issn.1673-8225.2010.12.013

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Does gelatin coating dacron patch containing source of tissue-type plasminogen activator gene transfer have effects on plasminogen activity in rabbit left atrium?

Liu Xiao-bin1, Zhang Kai-lun2, Jiang Xiong-gang2, Xia Jia-hong2, Xiang Dao-kang1, Li Cen1   

  1. 1 Department of Cardiovascular Surgery, Guizhou Provincial People’s Hospital, Guiyang  550001, Guizhou Province, China; 2 Department of Cardiovascular Surgery, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430020, Hubei Province, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Zhang Kai-lun, Doctor, Chief physician, Department of Cardiovascular Surgery, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430020, Hubei Province, China
  • About author:Liu Xiao-bin☆, Doctor, Chief physician, Department of Cardiovascular Surgery, Guizhou Provincial People’s Hospital, Guiyang 550001, Guizhou Province, China liuxiaobin930@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30571838*; Provincial Governor Foundation for Technology and Education Talent, No. 200763*

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Abstract:

BACKGROUND: Anticoagulant therapy after cardiac valve replacement puzzles cardiovascular surgeons; therefore, it has great value to develop mechanical valves that do not need anticoagulant therapy.
OBJECTIVE: To evaluate the effects of locally applied gelatin coating dacron patch containing source of tissue-type plasminogen activator (t-PA) gene transfer on plasmingen activity in left atrium and to provide a basis for construction of tPAcDNA gene valve.
METHODS: A total of 48 New Zealand white rabbits were divided into 3 groups, including transfected gene group (implant gelatin coating dacron patch containing tPAcDNA in left atrium), empty vector group (implant gelatin coating dacron patch containing empty vector) and blank group (implant gelatin coating dacron patch without gene). The specimens were harvested at 3 and 14 days after operation. The expression of tPA gene of some myocardium tissue in left atrium muscle was detected by RT-PCR and Western-Blot essay. The tPA activity of plasmingen tissues in left atrium and peripheral blood was measured by chromogenic substrate essay.
RESULTS AND CONCLUSION: At 3 and 14 days after operation, RT-PCR and Western-Blot confirmed the expression of tPAmRNA and the presence of tPA protein at the site of gene transfer, which was stained yellow brown. The tPA activity in experimental group was significantly higher than that in control groups (P < 0.05). The differences of tPA acitive have no significance. Gelatin coating dacron patch is feasible to be the carrier of gene transfection. Locally applied tPA gene can increase tPA activity in left aterium.

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