中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (35): 6536-6539.doi: 10.3969/j.issn.1673-8225.2010.35.019

• 骨与关节图像与影像 bone and joint imaging • 上一篇    下一篇

64排螺旋CT评估碱性成纤维细胞因子基因治疗兔缺血心肌的血管生成

龙开林，周  攀   

  1. 中南大学湘雅三医院放射科，湖南省长沙市  410013
  • 出版日期:2010-08-27 发布日期:2010-08-27
  • 作者简介:龙开林，男，1973年生，湖南省靖州县人，侗族，2008年中南大学毕业，主管技师，主要从事医学影像学方面的研究。 longkl328@126.com
  • 基金资助:

    湖南省卫生厅科研基金资助项目(B2009-065)。

Application of 64-slice CT imaging in the evaluation of basic fibroblast growth factor on angiogenesis in myocardial ischemia rabbits

Long Kai-lin, Zhou Pan   

  1. Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha  410013, Hunan Province, China
  • Online:2010-08-27 Published:2010-08-27
  • About author:Long Kai-lin, Technician-in-charge, Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China longkl328@126.com
  • Supported by:

    the Science and Technology Foundation of Health Department of Hunan Province, No. B2009-065*

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摘要：

背景：64排螺旋CT检查以无创性等优点作为冠状动脉狭窄介入治疗前的筛选，在诊断早期冠心病及评价心功能方面具有重要的临床价值。但目前尚无利用64排螺旋CT评估基因治疗效果的研究。
目的：应用64排螺旋CT评估碱性成纤维细胞因子基因对兔缺血心肌血管生成的影响。
方法：建立新西兰兔急性心肌缺血模型，模型稳定后分别心肌注射200 µL携带碱性成纤维细胞因子基因的聚酰胺-胺型纳米载体的磷酸盐缓冲液或磷酸盐缓冲液。4周后，行64排螺旋 CT显影观察梗死心肌和左室心肌面积，同时取心肌组织2%TTC染色观察每个层面的梗死心肌和左室心肌面积，并于高倍镜下计数缺血区域新生血管数目。
结果与结论：64排螺旋CT显像示治疗组梗死心肌容积百分比显著小于对照组(P < 0.05)。治疗组TTC染色梗死心肌容积小于对照组(P < 0.05)。治疗组单个高倍视野内的新生血管数显著高于对照组(P < 0.05)。结果表明聚酰胺-胺型载体介导的碱性成纤维细胞因子基因能诱导缺血心肌血管生成，64排螺旋 CT可以用来评估心肌缺血基因治疗效果。

关键词: 碱性成纤维细胞因子, 64排螺旋CT, 聚酰胺-胺型纳米载体, 心肌缺血, 基因治疗

Abstract:

BACKGROUND: 64-slice spiral CT provides noninvasive approach that can potentially expand the utility of coronary angiography. However, there are few reports concerning 64-slice spiral CT in evaluating therapeutic effect of gene therapy.
OBJECTIVE: To evaluate the effect of basic fibroblast growth factor (bFGF) on angiogenesis in myocardial ischemia using 64-slice spiral CT.
METHODS: New Zealand rabbits with acute myocardial ischemia were randomly assigned to the bFGF group and control group. Totally 200 μL buffer phosphate containing polyamidoamine carrying bFGF cDNA nano-carriers or buffer phosphate only was injected into ischemia myocardia. Four weeks after injection, 64-slice spiral CT imaging and immunohistochemistry were applied to examine the region of ischemia myocardial and increased density of capillaries.
RESULTS AND CONCLUSION: 64-slice spiral CT imaging demonstrated that, the percentage of ischemia myocardial region in the treatment group was obviously smaller than that of the control group (P < 0.05). Furthermore, density of capillaries and arteries were increased in the myocardial section of the treatment group (P < 0.05). The findings indicated that, gene therapy with PAMAM-bFGF induces arteriogenesis in the region of myocardial ischemia. 64-slice spiral CT can evaluate therapeutic efficacy of gene therapy for myocardial ischemia.

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