中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5186-5190.doi: 10.3969/j.issn.1673-8225.2010.28.013

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

RhoA/ROCK-I信号通路与增生性瘢痕成纤维细胞结缔组织生长因子的表达

戴丽冰，潘  姝，沈  雁，黄  粤，梁  蓉，康  宁，李叶扬，李孝建，谢有富，李  罡，张  琰   

  1. 暨南大学医学院第四附属医院，广州市红十字会医院，广州市创伤外科研究所，广东省广州市 510220
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 作者简介:戴丽冰，女，1970年生，广东省广州市人，汉族，2007年广州医学院毕业，医学实验主管技师，主要从事创伤医学基础研究。Libingdai@126.com
  • 基金资助:

    广东省中医药局科研项目( No: 2007401 )；广州市中医药、中西医结合科研项目( No: 2007A16 )；广州市科技局项目( No: 2008J1-C121)；广东省自然科学基金(No:9152800001000009 )；广州市医药卫生科技重点项目(No:2005-Zdi-03，2006-Zdi-08，2008-Zdi-08，2009-Zdi- 05，2009-Zdi-12)。

RhoA/ROCK-I signaling pathway and expression of connective tissue growth factor in hypertrophic scar fibroblasts 

Dai Li-bing, Pan Shu, Shen Yan, Huang Yue, Liang Rong, Kang Ning, Li Ye-yang, Li Xiao-jian, Xie You-fu, Li Gang, Zhang Yan   

  1. Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou  510220, Guangdong Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Dai Li-bing, Technician-in-charge, Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou 510220, Guangdong Province, China Libingdai@126.com
  • Supported by:

    the Administration of Traditional Chinese Medicine of Guangdong Province, No. 2007401*; Foundation of Traditional and Western Medicine, No. 2007A16*; Program of Science and Technology Bureau of Guangzhou, No. 2008J1-C121*; the Natural Science Foundation of Guangdong Province, No. 9152800001000 009*; Key Program of Medical Science of Guangzhou, No. 2005-Zdi-03*, 2006-Zdi-08*,2008-Zdi-08*, 2009-Zdi-05*, 2009-Zdi-12*

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1061

被引次数

15

摘要：

背景：前期实验表明增生性瘢痕中RhoA和ROCK-I基因表达较正常皮肤高，提示RhoA/ROCK-I信号通路可能参与了增生性瘢痕的发生，但其在病理性瘢痕中的作用尚不清楚。
目的：研究RhoA/ROCK-I信号通路在增生性瘢痕成纤维细胞结缔组织生长因子(connective tissue growth factor，CTGF)表达调控中的作用。
方法：分离培养人增生性瘢痕组织来源的成纤维细胞，应用转化生长因子β1及Rho激酶的特异抑制剂Y-27632对细胞进行干预实验。采用实时荧光定量PCR及免疫荧光细胞化学方法检测瘢痕成纤维细胞中RhoA，ROCK-I及CTGF mRNA与蛋白的表达。
结果与结论：给予转化生长因子β1后，增生性瘢痕成纤维细胞中RhoA，ROCK-I及CTGF mRNA与蛋白表达明显增多(P < 0.01)；而Y-27632能阻碍转化生长因子β1的作用；但单独给予Y-27632并不引起瘢痕成纤维细胞中RhoA，ROCK-I及CTGF的mRNA与蛋白表达改变。说明转化生长因子β1可通过RhoA/ROCK-I信号通路调控CTGF mRNA与蛋白的表达，即RhoA/ROCK-I信号通路参与了瘢痕成纤维细胞CTGF的表达调控，阻断RhoA下游通路是增生性瘢痕治疗靶点之一。

关键词: 增生性瘢痕, 成纤维细胞, RhoA, ROCK-I, 结缔组织生长因子

Abstract:

BACKGROUND: Our previous research has demonstrated that the expression of RhoA and ROCK-I in hypertrophic scar fibroblasts were greater than those in normal skins. These result suggested that RhoA/ROCK signaling pathway might take part in the hypertrophic scar formation. But these effects are still unclear in the hypertrophic scar formation.
OBJECTIVE: To study the effects of on expression of connective tissue growth factor (CTGF) in hypertrophic scar fibroblasts.
METHODS: Fibroblasts were obtained from human hypertrophic scars and cultured in the mediums. Transforming growth factor β1 (TGF-β1) and Y-27632 were used to intervene the cells. Fluorescent quantitative PCR assay and immunofluorescence cytochemistry were used to quantify the expression and secretion of RhocA, ROCK-I and CTGF in the hypertrophic scar fibroblasts.
RESULTS AND CONCLUSION: Upon TGF-β1 treatment, the expression and secretion of RhocA, ROCK-I and CTGF in the hypertrophic scar fibroblasts were significantly increased (P < 0.01). But these effects of TGF-β1 on ROCK-I and CTGF were inhibited in the TGF-β1+Y-27632 group. However, there were no expression changes after single Y-27632 treatment. This indicated that TGF-β1 can regulate the expression of CTGF via RhoA/ROCK-I signaling pathway, namely, RhoA/ROCK-I signaling pathway participate the expression regulation of CTGF in hypertrophic scar fibroblasts and block the downstream pathway, which may be one of the molecular mechanism of TGF-β1 in effecting hypertrophic scar formation.

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