中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3895-3899.doi: 10.3969/j.issn.1673-8225.2011.21.022

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

链霉素缓释复合降解膜植入损伤肌腱局部腱周结缔组织的变化

富  玲1，宋  宁1，苏学忠2，李娜然3，孙佩杰3，苏秋香3，杨晓霞4   

  1. 1沈阳医学院奉天医院手外科，辽宁省沈阳市  110024；2沈阳医学院，辽宁省沈阳市  110034；沈阳医学院，3形态中心，4病理教研室，辽宁省沈阳市  110034
  • 收稿日期:2011-01-13 修回日期:2011-04-13 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 杨晓霞，硕士，教授，硕士生导师，沈阳医学院医学应用技术学院，辽宁省沈阳市 110034 yangxx116@163.com
  • 作者简介:富玲★，女，1977年生，辽宁省抚顺市人，满族，2007年吉林大学毕业，硕士，主治医师，主要从事组织损伤修复研究。 Fuling033103311@sina.com

Connective tissue changes at the position of tendon injury following implantation of streptomycin combined with slow-releasing biodegradable membrane

Fu Ling1, Song Ning1, Su Xue-zhong2, Li Na-ran3, Sun Pei-jie3, Su Qiu-xiang3, Yang Xiao-xia4   

  1. 1Department of Hand Surgery, Fengtain Hospital, Shenyang Medical College, Shenyang  110024, Liaoning Province, China; 2Shenyang Medical College, Shenyang  110024, Liaoning Province, China; 3Morphology Center, 4Department of Pathology, Shenyang Medical College, Shenyang  110034, Liaoning Province, China
  • Received:2011-01-13 Revised:2011-04-13 Online:2011-05-21 Published:2011-05-21
  • Contact: Yang Xiao-xia, Master, Professor, Master’s supervisor, Department of Pathology, Shenyang Medical College, Shenyang 110034, Liaoning Province, China yangxx116@163.com
  • About author:Fu Ling★, Master, Attending physician, Department of Hand Surgery, Fengtain Hospital, Shenyang Medical College, Shenyang 110024, Liaoning Province, China Fuling033103311@sina.com

PDF

370

被引次数

7

摘要：

背景：国内外学者曾用生物、非生物及药物等方法，诸如生物膜、透明质酸钠、纤维素密封胶等材料进行大量肌腱修复防粘连研究，但到目前为止尚未取得突破性进展。
目的：观察肌腱损伤局部植入链霉素复合降解膜后腱周结缔组织的增生。
方法：切断SD大鼠跟腱后，随机数字表法分为3组，分别在肌腱断端缝合处植入链霉素复合缓释降解膜、维生素C复合缓释降解膜、空白缓释降解膜。术后90 d行肌腱损伤局部组织学观察、羟脯氨酸含量和生物力学指标检测。
结果与结论：链霉素复合缓释降解膜组肌腱缝合处内部的成纤维细胞、胶原纤维均较其腱周围、维生素C复合缓释降解膜组、空白缓释降解膜组多；腱缝合处肌腱周围多为正常结构的疏松结缔组织，很少有增生的结缔组织长入肌腱内部；肌腱与周围组织分界清晰，最大抗拉强度、羟脯氨酸含量明显优于其他两组。表明链霉素复合缓释降解膜通过抑制腱周结缔组织增生，防止腱周结缔组织增生长入腱内，从而减轻或防止粘连形成。

关键词: 肌腱损伤, 链霉素, 缓释降解膜, 维生素C, 粘连

Abstract:

BACKGROUND: Domestic and foreign scholars have used biological, non-biological, drug and other methods, such as biofilm, sodium hyaluronate, cellulose sealant, to perform a large number of studies on anti-adhesion tendon repair. However, there is no breakthrough up to now.
OBJECTIVE: To observe the proliferation of connective tissues after implantation of streptomycin combined with slow-releasing biodegradable membrane.
METHODS: After tendon cutting, SD rats were randomly divided into three groups: In group A, streptomycin combined with slow-releasing biodegradable membrane was implanted into the sutured site of Achilles tendon; in group B, vitamin C combined with slow-releasing degradable membrane were implanted; in group C, single slow-releasing degradable membrane was used. After 90 days, histological observation was performed for tendon injury as well as tractus hydroxyproline content and biomechanics indexes were measured.
RESULTS AND CONCLUSION: In the group A, there were more fibroblasts and collagen fibers in the sutured site of Achilles tendon than its surrounding tissues, group B and group C. Surrounding the sutured site, many loose connective tissue with normal structure could be seen, but few hyperplastic connective tissues grew into the tendon. A clear demarcation existed between the tendon and its peripheral tissues. The content of hydroxyproline and the ultimate tensile strength in group A were higher than those of groups B and C. The findings show that streptomycin combined with slow-releasing biodegradable membrane can alleviate or prevent tendon adhesion through inhibiting peripheral connective tissue proliferation and preventing connective tissue to grow into the tendon.

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