Bone cement particles are important to the proliferative capacity of human nucleus pulposus cells

doi:10.3969/j.issn.2095-4344.2013.12.003

Abstract

Abstract:

BACKGROUND: Vertebroplasty and kyphoplasty are widely used to treat osteoporotic vertebral fractures. Cement leakage into the intervertebral disc is one of common postoperative complications. The effect of cement leakage on intervertebral disc cells is not clear.
OBJECTIVE: To investigate the influence of bone cement particles on the proliferative capacity of human nucleus pulposus cells.
METHODS: Human nucleus pulposus cells were treated with bone cement particles including calcium phosphate cement or polymethylmethacrylate particles at the concentration of 0.001%, 0.01%, 0.1%, 0.5% and 1.0% (v/v). Routinely cultured human nucleus pulposus cells served as controls. Cell viability in different groups was tested with Cell Counting Kit-8.
RESULTS AND CONCLUSION: Calcium phosphate cement particles of different concentrations had no significant effect on the proliferative capacity of human nucleus pulposus cells. Polymethylmethacrylate particles at the concentration of 0.001% and 0.01% (v/v) did not have a significant impact on the cell viability of normal human nucleus pulposus cells; but when the concentration was above 0.1% (v/v), polymethylmethacrylate particles could significantly inhibit the proliferation activity of the nucleus pulposus cells in a dose-dependent manner as compared with the control group (P < 0.05). After 3 or 6 days of treatment with the polymethylmethacrylate particles, the inhibition effect on human nucleus pulposus cells proliferation was increased gradually with increasing concentration of the particles; when the polymethylmethacrylate particles were at the concentration of 0.1%, 0.5% and 1.0 % (v/v), the inhibition effect on the human nucleus pulposus cells proliferation was strengthened depending on the duration of particle treatment.

Key words: biomaterials, tissue-engineered bone materials, calcium phosphate cement particles, polymethylmethacrylate particles, nucleus pulposus cells, cell proliferation, the National Natural Science Foundation of China, biomaterial photographs-containing paper

CLC Number:

R318

Mao Hai-qing, Geng De-chun, Zhu Xue-song, Zou Jun, Yang Hui-lin. Bone cement particles are important to the proliferative capacity of human nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(12): 2108-2115.

Figures/Tables 9

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