Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3540-3544.doi: 10.3969/j.issn.1673-8225.2012.19.025

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Bone marrow stem cells transplantation for treatment of delayed encephalopathy after acute carbon monoxide poisoning in rats

Xing Hong-xia1, 2, Yin Chuang3, Liu Sheng4, Zhu Yuan-kai2, Shi Li-jin2, Tian Xiao-jun2, Zhang Bo-ai1, Wang Yu-mei2   

  1. 1Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou  450001, Henan Province, China; 2First Department of Neurology, 4Second Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical College, Weihui  453100, Henan Province, China; 3Department of Neurology, the Third Affiliated Hospital of Xinxiang Medical College, XinXiang  453003, Henan Province, China
  • Received:2011-09-10 Revised:2011-10-03 Online:2012-05-06 Published:2012-05-06
  • Contact: Zhang Bo-ai, Professor, Chief physician, Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, Henan Province, China zhangboaidoctor@163.com Wang Yu-mei, Chief physician, No.1 Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China yumei2628@163.com
  • About author:Xing Hong-xia☆, Studying for doctorate, Associate chief physician, Associate professor, Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, Henan Province, China; First Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China xhxwh02@163.com; xhxwh02@gmail.com
  • Supported by:

    Established Program in 2007 of Henan Science and Technology Bureau, No. 072102310077*

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Abstract:

BACKGROUND: The therapy of delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) in our country mainly depends on drug or hyperbaric oxygen. But the treatment has the characters of longer course, lower effect and higher cost.
OBJECTIVE: To observe the changes of mature neurons and cell apoptosis before and after bone marrow mesenchymal stem cells (BMSCs) transplantation for treatment of DEACMP.
METHODS: SD rats were randomly divided into three groups: sham operation group, control group and transplantation group. The rat model with DEACMP was established and intracerebral injection of allogeneic BMSCs was performed in the transplantation group at 0, 3, 5, 12, 24, 72 hours and 1 week after modeling, and cell transplantation was not performed in the control group. In the sham operation group, the external carotid artery was tied off and the PBS was injected instead of cell suspension. 
RESULTS AND CONCLUSION: The expression of neuron-core protein (NeuN) in rat brain in the transplantation group at 3, 6,12 and 24 hours were significantly higher than that in the control group and sham operation group (P < 0.01). There was no significant difference in NeuN expression at 3, 6, 12 and 24 hours (P > 0.05). The average absorbance value of apoptosis in the transplantation group across 0-hour, 3-hour and 6-hour three time points was significantly lower than that in the sham operation group and other transplantation groups (P < 0.05). The average absorbance value of apoptosis in 1 week group was significantly lower than that in 2-week, 3-week and 4-week groups (P < 0.05). Transplantating BMSCs into the DEACMP rat models through the carotid artery could increase the quantity of mature neurons, and the underlying mechanism has relationship with apoptosis reduction. The best time for transplantation was between the 0 hour and the 24 hours after falling ill and the positive effect was significant at 1 week after transplantation.

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