Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3457-3461.doi: 10.3969/j.issn.1673-8225.2012.19.008

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Effect of bone marrow-derived mesenchymal stem cell transplantation on the expression of MAG, OMgp in perihematomal tissue in rats with intracerebral hemorrhage

Yan Yu, Wang Yan-lin, Song Bo, Zhang Hui-li, Qin Jie, Wei Chen-ming, Wang Hai-li, Xu Yu-ming   

  1. Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Received:2012-01-01 Revised:2012-02-27 Online:2012-05-06 Published:2012-05-06
  • Contact: Xu Yu-ming, M.D., Professor, Chief physician, Doctoral supervisor, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China xuyuming@ zzu.edu.cn
  • About author:Yan Yu★, Studying for master’s degree, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China yanyuyes@163.com
  • Supported by:

    the Science and Technology Major Project of the Ministry of Science and Technology of China, No. 091100910800*

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Abstract:

BACKGROUND: Axon regeneration of central nervous system after injury is hard, which is associated with the elevated expression of the axon growth inhibitors: Nogo-A, MAG, OMgp around the lesions.
OBJECTIVE: To investigate the effects of intravenous administration of bone marrow-derived mesenchymal stem cells (BMSCs) on the expression of MAG and OMgp in perihematoma tissue in rats with intracerebral hemorrhage (ICH).
METHODS: BMSCs were isolated and cultured using the whole bone marrow adherent method. A rat model of ICH was established by stereotaxical injection of collagenase IV into the striatum. Sprague-Dawley (SD) rats were randomly divided into sham-operated group, ICH group (model) and ICH plus BMSCs intervention group (BMSCs group). Rats in the BMSCs group were transfused with BMSCs (1×107/rat) via tail vein at 24 hours after surgery. On days 1, 3, 7, 14 after surgery, neurological function tests were performed among all three groups and animals were sacrificed at the corresponding time points for detecting the expression of MAG, OMgp by immunohistochemical method.
RESULTS AND CONCLUSION: No obvious neurological function deficit was observed in sham-operated group on days 1, 3, 7, 14 after surgery. In the model group, the degree of neurological function deficit reached the peak on day 1 after surgery, lasted to day 3, recovery could be observed on day 7, and much more recovery could be seen on day 14. Compared to model group, the BMSCs group had significantly lower mean neurological function rating scores on days 3, 7,14 after surgery (P < 0.05). The mean expression levels of MAG, OMgp in neurons and gliocytes in perihematoma tissue in the model and BMSCs group were both significantly higher than that in the sham-operated group on days1, 3, 7,14 after surgery; meanwhile, the BMSCs group had significantly lower mean expression levels than model group on days 3, 7,14 after surgery (P﹤0.05). Transplantation of BMSCs could significantly promote the neurological function recovery of a rat model of ICH, which might be associated with its downregulation of MAG, OMgp in perihematoma tissue.

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