Abstract:

BACKGROUND: Human leukocyte antigen (HLA) is closely related with immunogenetics and immunobiology, its types influence the outcome of clinical transplantation and confer susceptibility to a wide range of non-infectious diseases.
OBJECTIVE: To investigate the genetic polymorphism of HLA-A locus gene in Jilin Han populations and to compare the distribution characteristic of the allele frequency with that in other populations.
METHODS: A total of 2 196 blood samples from Jilin Han populations were analyzed by sequence based typing. Then the sequences encompassing exons 2, 3 and 4 for HLA-A gene were analyzed by direct sequencing of polymerase chain reaction products. The allele frequency distribution of HLA-A in this populations was compared with that in other populations.
RESULTS AND CONCLUSION: A total of 42 different HLA-A alleles were detected, and among them, 3 HLA-A alleles with frequencies were higher than 5%, they were HLA-A*02:01(8.5%), HLA-A*11:01(8.2%) and HLA-A*24:02(7.3%). The total frequency of the 3 alleles was 24.0%. At the same time, there were 39 kinds of HLA-A allele with frequencies lower than 0.5%; the total frequency of these alleles was 76.0%. There were some differences of frequency distribution of HLA-A allele in Jilin Han population when compared with American Asian, the Chinese populations from Hong Kong and Japanese, respectively. The results show the geographical characteristic of HLA-A distribution in Jilin Han populations.