BACKGROUND: The liver biological scaffold made by decellularization technology is the new technology to alleviate the shortage of donor; the optimization of the process to liver tissue decellularization is the new topic.
OBJECTIVE: To establish the liver biological scaffold preserving the liver three-dimensional structure and the intact vascular system by decellularization technology, then, to realize the in vitro cell replantation of liver biological by scaffold independent designed circulating-perfusion culture device. 
METHODS: The cannula in the portal veins were attached to the pump and was perfused sequentially with TritonX-100 and sodium dodecyl sulfate, and then was perfused with phosphate buffered saline to wash out the residual detergent. The liver biological scaffold was cultured with HepG2 cells through the circulating-perfusion culture device and the expression of the cells in the liver biological scaffold was observed.
RESULTS AND CONCLUSION: After the perfusion of the detergent, the scaffold was retained the transparent structure of the liver three-dimensional structure. Hematoxylin-eosin staining and scanning electron microscope reveled that the cellular components were completely removed. Masson’s Trichrome staining showed that there was a large amount of collagen fibers; the immunohistochemical results confirmed that fibronectin and laminin preserved intactly. Expression of cell albumin and the volume of the urea synthesis in the circulating-perfusion culture were significantly increased compared with plate culture. It indicate that the decellularized liver biological scaffold can be used as the basis materials of in vitro liver tissue reconstruction, and the dynamic circulating-perfusion culture method can achieved the cell replantation in the scaffold.