Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2955-2958.doi: 10.3969/j.issn.1673-8225.2012.16.025

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Cytocompatibility and cytotoxicity of coral hydroxyapatite in the periodontal tissue engineering

Lu Hong1, Tian Yu2, Wu Zhi-fen1   

  1. 1Department of Periodontology and Oral Medicine  Diseases, 2Department of Operative Dentisty and Endodonties, School of Stomatology, the Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China
  • Received:2012-01-09 Revised:2012-02-13 Online:2012-04-15 Published:2012-04-15
  • Contact: Tian Yu, Doctor, Associate professor, Associate chief physician, Department of Operative Dentistry and Endodonties, School of Stomatology, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China tianyu@fmmu.edu.cn
  • About author:Lu Hong☆, Doctor, Attending physician, Lecturer, Department of Periodontology and Oral Medicine Diseases, School of Stomatology, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China lu_hong@yahoo.com
  • Supported by:

    the Science and Technology Development Program of Shaanxi Province, No. 2010K12-02-03*, 2010K01-193*

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Abstract:

BACKGROUND: With excellent biocompatibility and degradability, porous coral hydroxyapatite can provide the enough three-dimensional space for the adherence and proliferation of seed cells, which is beneficial for the vascularization and the infiltration of nutrients.
OBJECTIVE: To investigate the cytocompatibility and cytotoxicity of coral hydroxyapatite so as to evaluate the feasibility of coral hydroxyapatite as a scaffold of periodontal tissue engineering.
METHODS: Human periodontal ligament cells (HPDLCs) cultured in vitro were collected and seeded on the three-dimensional framework of coral hydroxyapatite. The HPDLCs growth on coral hydroxyapatite scaffolds was observed by cell counting and scanning electronic microscope. Compared with the negative and positive controls, the influences of coral hydroxyapatite extracts at dilution concentrations of 100%, 50%, 10%, 1% on cell proliferation and differentiation were observed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and alkaline phosphatase activity assay.
RESULTS AND CONCLUSION: The cell counting results showed that human periodontal ligament cells adhered and proliferated on the three-dimensional framework of coral hydroxyapatite, and the flourishing growth of cells in the porous coral hydroxyapatite scaffolds was observed by scanning electronic microscope. The cytotoxicity detection showed that the different concentrations of coral hydroxyapatite extracts had no influences on the cell proliferation and differentiation. Then the porous coral hydroxyapatite was proved to be feasible as the periodontal tissue engineering scaffold with satisfactory cytocompatibility and with no cytotoxicity.

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