Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2875-2878.doi: 10.3969/j.issn.1673-8225.2012.16.006

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Gold nanochain and anti-epidermal growth factor receptor/Au near-infrared hyperthermia can start apoptosis pathway

Cong Lin-hai, Peng Shu-kun, He Xiao-guang, Yang Yi-bing   

  1. Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2012-01-09 Revised:2012-02-08 Online:2012-04-15 Published:2012-04-15
  • Contact: author: He Xiao-guang, Doctor, Professor, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China Conglinhai2008@ 126.com Peng Shu-kun, Technician in charge, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No.81160324/H1625*

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Abstract:

BACKGROUND: Gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.
OBJECTIVE: To evaluate the killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugates in near-infrared hyperthermia on human laryngeal cancer Hep-2 cells and to explore relevant apoptosis pathway.
METHODS: Human laryngeal squamous carcinoma Hep-2 cells were extracted and intervened in gold nanochain or anti-EGFR/Au solution. Then, the Hep-2 cells were exposed to the near-infrared laser irradiation of 8 W/cm2 (808 nm) for       6 minutes. The cells cultured alone in 1640 culture medium or only exposed to near-infrared laser were as controls.
RESULTS AND CONCLUSION: Under transmission electron microscope, the Hep-2 cells cultured in the gold nanochain solution after hyperthermia were significantly damaged, and the cells in the anti-EGFR/Au solution after hyperthermia were damaged worst. The Western blot detection exhibited that the expressions of heat-shock protein 70 and pro-apoptotic protein Bax were significantly increased, while the expression of anti-apoptotic protein Bcl-2 was obviously decreased exposed to gold nanochain and anti-EGFR/Au near-infrared hyperthermia. These suggest that gold nanochain and anti-EGFR/Au near-infrared hyperthermia can kill human laryngeal cancer Hep-2 cells via apo-ptosis pathway activation.

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