BACKGROUND: The mutation of DNA polymerase β (DNA pol β) has been found in esophageal carcinoma cells.
OBJECTIVE: To analyze the effect of alternariol on the sequence of DNA pol β in NIH/3T3 cell line and to study the mutation mechanism of alternariol
METHODS: The NIH/3T3 cell line was injected with different concentrations of alternariol (2, 4, 6, 8 μmol/L) and the control group was set. DNA pol β gene was amplified from the total RNA of the cells using reverse transcription-PCR, then cloned with pGEM-T vector and sequenced with T-A cloning technique, and the sequence was analyzed.
RESULTS AND CONCLUSION: The sequence of DNA pol β was not changed in 2 μmol/L alternariol group, and there was one mutation point in 4 μmol/L alternariol group and two mutation points in 8 and 16 μmol/L alternariol group. There was a same mutation point in these three groups. Alternariol can induce point mutation of DNA pol β gene in NIH/3T3 cell line. Higher concentration of alternariol, more points of DNA pol β gene mutation. The active mutation point exists.