Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (12): 2145-2148.doi: 10.3969/j.issn.1673-8225.2012.12.013

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Apoptosis of human laryngeal cancer cells induced by gold nanochain and anti-epidermal growth factor receptor/Au conjugate under near-infrared hyperthermia 

Cong Lin-hai, Peng Shu-kun, He Xiao-guang, Yang Yi-bing   

  1. Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Received:2011-11-19 Revised:2011-12-31 Online:2012-03-18 Published:2012-03-18
  • Contact: author: He Xiao-guang, Doctor, Professor, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China conglinhai2008@ 126.com Peng Shu-kun, Technician In charge, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China
  • Supported by:

     the National Natural Science Foundation of China, No. 81160324/H1625*

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Abstract:

BACKGROUND: The gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.
OBJECTIVE: To evaluate the growth inhibition and heat killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugate on human laryngeal cancer Hep-2 cells under near-infrared hyperthermia.
METHODS: The Hep-2 cells in the logarithmic growth phase were collected and divided into blank control group, irradiation group, gold nanochain irradiation group and anti-EGFR/Au irradiation group. After added with different culture medium, the near-infrared laser irradiation was performed in each group. Then the apoptosis of Hep-2 cells in different experimental groups was observed after the near-infrared laser irradiation.
RESULTS AND CONCLUSION: Inverted microscope observation showed that the Hep-2 cells in the blank control and irradiation groups were still active and the Hep-2 cells in the gold nanochain irradiation group were damaged significantly, while the Hep-2 cells in anti-EGFR/Au irradiation group were damaged more serious. Flow cytometry analysis showed that the Hep-2 cells in the gold nanochain irradiation and anti-EGFR/Au irradiation groups had a varying degree of apoptosis and the increasingly heavy damage with the time. The gold nanochain irradiation can kill Hep-2 cells and the anti-EGFR/Au irradiation has a better effect in a time-depended manner.

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