MRI tracking of superparamagnetic iron oxide-labeled rabbit bone marrow mesenchymal stem cells for treatment of femoral head avascular necrosis

doi:10.3969/j.issn.2095-4344.2014.10.013

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cell (BMSC) transplantation is one of the developmental directions in the treatment of femoral head necrosis. In recent years, the use of superparamagnetic iron oxide (SPIO) nanoparticles labeled target cells traced by MRI imaging method has become the focus of the study.
OBJECTIVE: To observe the in vivo MRI tracking and the curative effects of SPIO-labeled BMSC transplantation on rabbit femoral head necrosis.
METHODS: SPIO-labeled BMSCs, unlabeled BMSCs, and normal saline were injected in situ into the necrotic femoral head of rabbits. Following MRI dectection, the image changes of transplanted BMSCs marked by SPIO were observed among the three scanning sequences of SE T2WI, FSE T2WI and GRE T2*WI. Meanwhile, the area percentage of newly formed bone trabecula in the defect samples under high power lens were observed and calculated for statistical analysis.
RESULTS AND CONCLUSION: In situ cell transplantation group showed the emerging and extinctive time of the decreased-signal region was different among the three scanning sequences of SE T2WI, FSE T2WI and GRE T2*WI. It was found that the decreased-signal region of the MRI scanning sequences was the target of the present experiment. No obvious signal change occurred in the control side. After 6 weeks of transplantation, the area percentage of newly formed bone trabecula in the defect samples showed no difference in SPIO-labeled and unlabeled BMSC transplantation groups (P > 0.05), but it was higher than that in the control side (P < 0.01). The SPIO-labeled BMSCs and unlabeled BMSCs are shown to have the same effects in the treatment of femoral head necrosis. The SPIO-labeled BMSCs can be observed obviously by MRI detection in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, femur head necrosis, magnetic resonance imaging

CLC Number:

R394.2

Meng Zeng-dong, Li Lei, Hu Biao, Lei Yun-kun, Liu Wei, Tang Xu. MRI tracking of superparamagnetic iron oxide-labeled rabbit bone marrow mesenchymal stem cells for treatment of femoral head avascular necrosis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1560-1565.

Figures/Tables 3

以上MRI检测研究显示，随着时间的延长，股骨头坏死的高信号区随着SPIO标记的骨髓间充质干细胞与未标记的骨髓间充质干细胞移植治疗而信号逐渐减低，6周时可见坏死区信号与周围正常骨组织信号基本一致，表明坏死的股骨头已得到修复；同时，阴性对照组的股骨头坏死的高信号区在6周后无明显减低表现，现明坏死的股骨头未得到修复。 2.3 移植后组织学观察超顺磁性氧化铁标记的骨髓间充质干细胞移植侧：可见骨髓基质干细胞出现于坏死骨小梁旁边，呈“极化现象”，骨小梁附加骨及新生类骨质大量出现，缺损区内有少量的炎症细胞，边缘有较多的成骨细胞，并有类骨质及骨小梁产生，骨髓增生及骨修复大量进行，缺损区出现大量骨髓组织及成骨细胞，骨髓增生及纤维化并存，骨修复及骨吸收互见(图3A，B)。未标记的骨髓间充质干细胞移植侧：骨小梁附加骨及新生类骨质大量出现，可见骨髓基质干细胞出现于坏死骨小梁旁边，呈“极化现象”。缺损区内有少量的炎症细胞及成骨细胞，并有类骨质及骨小梁产生，可见骨髓增生及骨修复，缺损区出现较多量的骨髓组织及成骨细胞，骨髓增生及纤维化并存，骨修复及骨吸收可见(图3C，D)。生理盐水移植侧：软骨细胞可见少量增生和爬行修复，软骨面毛躁，部分剥脱，关节面部分塌陷，骨细胞及骨髓组织可见坏死，少数骨小梁变细，出现吸收和骨折，骨陷窝空虚，骨小梁紊乱，缺损区边缘可见少许新生骨小梁，缺损区出现骨髓组织，软骨下骨修复旺盛，骨髓增生以及骨修复存在(图3E，F)。 2.4 统计学分析移植后6周，实验1组+实验2组的超顺磁性氧化铁标记的骨髓间充质干细胞移植侧与实验2组+阴性对照组未标记的骨髓间充质干细胞移植侧高倍镜下缺损标本边缘新生骨小梁面积百分比为(19.31±2.82)%，(20.87±1.55)%，两组间的差异无显著性意义(P > 0.05)，均明显高于实验1组+阴性对照组生理盐水移植侧[(2.96± 1.25)%]，差异有显著性意义(P < 0.01)。"

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