Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1871-1874.doi: 10.3969/j.issn.1673-8225.2012.10.035

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Effect of shear stress on the copper-zinc superoxide dismutase gene expression and activity in human endothelial progenitor cells 

Yang Zhen1, Lai Guang-hua1, Xia Wen-hao1, Luo Chu-fan2, Wang Jie-mei1, Chen Long1, Liao Xin-xue1, Jin Ya-fei1, Tao Jun1   

  1. 1Department of Hypertension & Vascular Disease; 2Department of Cardiac Intervention, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-09-05 Revised:2011-11-01 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Tao Jun, M.D., Professor, Doctoral supervisor, Department of Hypertension & Vascular Disease, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China taojungz@yahoo.com
  • About author:Yang Zhen☆, M.D., Attending physician, Department of Hypertension & Vascular Disease, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China yangzhen107107@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30770895*, u0732002*, 30800215*; Doctoral Launching Project of Natural Science Foundation of Guangdong Province, No. 84510089010007 93*; Science and Technology Development Program of Guangzhou Province, No. 2007Z3-E0241*

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Abstract:

BACKGROUND: Shear stress is an important non-pharmacological method to regulated endothelial progenitor cells (EPCs). However, the effect of shear stress on the anti-oxidative activity of EPCs is not clear.
OBJECTIVE: To observe the effect of shear stress on the copper-zinc superoxide dismutase (Cu/Zn-SOD) gene expression and activity in EPCs, in order to investigate the regulatory effects of shear stress on the EPCs function.
METHODS: The peripheral blood mononuclear cells of healthy adult were inducted into EPCs. Then, they were divided into four different experimental groups which included stationary group, low-flow shear stress group (0.05 mN /cm2), medium-flow shear stress group (0.15 mN/cm2) and high-flow shear stress group (0.25 mN/cm2).
RESULTS AND CONCLUSION: The peripheral blood mononuclear cells were differentiated into EPCs. They presented typical “spindle-shaped” appearance, and were positively labeled by acetylated-LDL, lectin, FLK-1 and VWF. The Cu/Zn-SOD activity of human EPCs in shear stress treatment groups was higher than that in stationary group. The higher the shear stress, the higher the Cu/Zn-SOD activity of human EPCs. Quantitative real-time PCR indicated that shear stress could increase the CuZn-SOD mRNA expression of EPCs, the higher the shear stress, the stronger the CuZn-SOD mRNA expression of EPCs. In vitro shear stress could upregulate the gene expression of Cu/Zn-SOD in EPCs, increase the Cu/Zn-SOD activity in human EPCs, which contributes to enhance the anti-oxidative activity of EPCs. Therefore, enhanced shear stress within the physiological range could regulate the function of EPCs, which can act as an important strategy of enhancing their therapeutic approach for vascular endothelial injury.

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