Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1721-1724.doi: 10.3969/j.issn.1673-8225.2012.10.003

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Effects of 17 beta-estradiol on osteogenic differentiation of rat primary cultured bone marrow mesenchymal stem cells

Han Na1, Kou Yu-hui2, Wang Tian-bing2, Yin Xiao-feng2, He Pei-ying1, Zhang Pei-xun2   

  1. 1Institute of Clinical Molecular Biology, 2Department of Orthopedics and Traumatology, Peking University People’s Hospital, Beijing 100044, China
  • Received:2012-01-11 Revised:2012-01-31 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Zhang Pei-xun, Department of Orthopedics and Traumatology, Peking University People’s Hospital, Beijing 100044, China zhangpeixun@126.com
  • About author:Han Na☆, M.D., Research Assistant, Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China hannaqd@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30973066*, 31040043*; the Natural Science Foundation of Beijing, No. 7113170*; Doctoral Fund of Ministry of Education of China, No. 20090001120096*; Peking University People’s Hospital Research and Development Funds, No. RDB2010-30*

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Abstract:

BACKGROUND: The estrogen receptor exists in bone marrow mesenchymal stem cells and estrogen promotes bone formation by regulating the proliferation and differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To explore the effects of 17β-estradiol on the osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) and the underlying mechanism.
METHODS: MSCs were separated, purified, cultured and expanded. Different concentrations of 17β-estradiol was administrated to MSCs for some period. The level of type I collagen in culture medium was detected by Elisa method, and the expression of Runx2 mRNA and protein was analyzed by RT-PCR or Western blotting methods, respectively.
RESULTS: Compared with the control group, type I collagen expression in the MSCs was significantly increased on the 5th day after administration of 0.001, 0.01, 0.1 nmol/L 17β estradiol (P < 0.05) and it maintained high expression on the 7th day (P < 0.05). Runx2 mRNA expression increased in a dose-dependent manner on the 5th and 7th days after administration of 17 β-estradiol
(P < 0.05). These findings suggest that 17β-estradiol can induce the osteogenic differentiation of MSCs, which occurs possibly by upregulation of Runx2 expression.

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