Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1451-1454.doi: 10.3969/j.issn.1673-8225.2012.08.027

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Effects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of streptococcus mutans 

Yan Wen-juan, Xu Shu-jun, Xie Miao-miao, Wu Bu-ling   

  1. Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-10-22 Revised:2011-12-16 Online:2012-02-19 Published:2012-02-19
  • Contact: Wu Bu-ling, Chief physician, Doctoral supervisor, Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China Wubuling@ yahoo.com.cn
  • About author:Yan Wen-juan☆, Doctor, Lecturer, Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China ywj918@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No.81100747*

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Abstract:

BACKGROUND: The preliminary study has confirmed the existence of cyclic dimeric guanosinemonophosphate (c-di-GMP) signaling pathway in streptococcus mutans. The pathway mediates the streptococcus mutans biofilm formation and in vitro adhesion to enamel surface.
OBJECTIVE: To analysis the effects of c-di-GMP on the biological properties of streptococcus mutans by gene chip technology.
METHODS: Exogenous c-di-GMP was used to interfere streptococcus mutans UA159. The total RNA was extracted, the total RNA of standard strain was as control, and then hybridized with streptococcus mutans whole-genome chips. The different genes were filtered.
RESULTS AND CONCLUSION: Expressions of 9 genes (glgA, glgB, msmF, gftA, lacG, lepB, rgpAc, bacC, apt6) were up-regulated while six genes (Hrc, ProC, HprT, Pdp, Glk, cdsA) expressions were down-regulated after exogenous c-di-GMP interfering. Differences genes were mainly related to the biological characteristics, such as cell tropism, biofilm formation, signal transduction, and energy metabolism. It showed that the c-di-GMP could affect the cariogenic ability of streptococcus mutans.

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