Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1345-1348.doi: 10.3969/j.issn.1673-8225.2012.08.004

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R318Combination application of human amniotic mesenchymal cells and poly (L-lactic acid)/ hydroxyapatite membranes for construction of cells/scaffold complexes in bone tissue engineering 

Zhong Jin-sheng1, Mei Fang2, Qi Wei-hong3, Yang Xiao-ping4   

  1. 1Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing  100081, China; 2Department of Histology and Embryology, Peking University Health Science Center, Beijing  100191, China; 3Department of Obstetrics and Gynecology, Beijing Hospital, Beijing  100730, China; 4The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemistry and Technology, Beijing  100029, China
  • Received:2011-12-23 Revised:2012-01-11 Online:2012-02-19 Published:2012-02-19
  • Contact: Mei Fang, Lecturer, Department of Histology and Embryology, Peking University Health Science Center, Beijing 100191, China meifang@ bjmu.edu.cn
  • About author:Zhong Jin-sheng☆, Doctor, Attending physician, Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China zhongjs@263.net
  • Supported by:

    the National Natural Science Foundation of China, No. 30471907*

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Abstract:

BACKGROUND: Previous study has shown that electrospun poly (L-lactic acid)/hydroxyapatite (PLLA/HA) membranes are suitable for cellular adhesion and proliferation.
OBJECTIVE: To investigate the feasibility of the human amniotic mesenchymal cells (hAMCs) and PLLA/HA membranes constructing the complex of seed cells/scaffold in bone tissue engineering.
METHODS: Effect of leaching liquor from the PLLA and PLLA/HA membranes on the proliferation of hAMCs was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay method. The third passage of hAMCs were cultured in the osteogenic induce media containing PLLA and PLLA/HA membranes, and then were detected by histological and immunofluorescence cytochemical staining.
RESULTS AND CONCLUSION: The leaching liquor of the PLLA and PLLA/HA had no cytotoxicity on the hAMCs. After seeded onto two kinds of the membranes, hAMCs proliferated actively and calcified nodules were observed. Cells in calcified nodules were shown positive expression of collagen type Ⅰ and alkaline phosphatase, and the number and maturity of the calcified nodules in the PLLA/HA group were better than those of the PLLA group. It is showed that the hAMCs and PLLA/HA can construct cells/scaffold complex and show a great potential of application in bone tissue engineering.

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