Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 863-866.doi: 10.3969/j.issn.1673-8225.2012.05.025

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Comparison of two methods of tendon preservation at normal temperature in glycerine

Han Bing, Song Yi-ping, Wang He-hong, Chen Shuo, Tong Xun, Zhao Ri-guang, Sun Yi-yan, Feng Hui   

  1. Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou  221004, Jiangsu Province, China
  • Received:2011-07-30 Revised:2011-10-20 Online:2012-01-29 Published:2012-01-29
  • Contact: Song Yi-ping, Chief physician, Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China songyipingjsxz@163.com
  • About author:Han Bing★, Master, Physician, Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China hanbingjsxz@163.com
  • Supported by:

    “Eleventh Five-Year” Plan Project of Medical Science and Technology Research of Nanjing Military Region, No.06z17*

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Abstract:

BACKGROUND: The greatest degree to obtain the biological activity of tendon is the conditions for allograft tendon transplantation.
OBJECTIVE: To select the best tendon preservation method at room temperature.
METHODS: The rabbit tendons were randomly divided into four groups using the aseptic technique: Control group of fresh tendon, normal saline group, anhydrous glycerol group Ⅰ(tendons were preserved in anhydrous glycerol after gradient dehydration), anhydrous glycerol group Ⅱ (tendons were preserved in anhydrous glycerol directly). Tendons in each group were preformed with examination at 2, 4 and 7 months.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that the cell integrity of anhydrous glycerol group Ⅰ was higher than that of anhydrous glycerol group Ⅱ. Electron microscope observation showed that the morphology of most tendon cells in anhydrous glycerol group Ⅰ was normal, the structure of tendon tissue was integrated; After the tendons were preserved in anhydrous glycerol group Ⅱ for 4 and 7 months, the nucleus were coagulation, condensation and collapse-like. The activity of superoxide dismutase in anhydrous glycerol group Ⅰ was obviously higher than that in the anhydrous glycerol group Ⅱ. It indicates that the tendon preformed with gradient dehydration can be preserved in anhydrous glycerol effectively.  

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