Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (3): 443-448.doi: 10.3969/j.issn.1673-8225.2012.03.014

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Bone marrow mesenchymal stem cells combined with small intestinal submucosa to construct tissue-engineered skin for repair of diabetes mellitus induced full-thickness skin defects

Wang Shao-yun1, Wu Di2, Zhang Li3, Zhu Xiao-song2, Yang Hao2, Li Ci-hui4, Wu Shu4, Li Shi-he2, Dong Jian5   

  1. 1Department of Orthopedics, Third Affiliated Hospital, Kunming Medical University, Kunming 650000, Yunnan Province, China; 2Department of Orthopedics, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China; 3Function Department, Basic Medical School of Kunming Medical University, Kunming  650031, Yunnan Province, China; 4Beijing Datsing Bio-Tech, Beijing 100000, China; 5Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-05-21 Revised:2011-06-16 Online:2012-01-15 Published:2012-01-15
  • Contact: Dong Jian, Doctor, Doctoral supervisor, Centre of Biotherapy, First Affiliated Hospital, Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Wang Shao-yun☆, Doctor, Physician, Department of Orthopedics, Third Affiliated Hospital, Kunming Medical University, Kunming 650000, Yunnan Province, China Reinhard007550@ 163.com
  • Supported by:

    United Foundation of Yunnan Science and Technology Bureau & Kunming Medical University, No. 2007C0002R*

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Abstract:

BACKGROUND: Small intestinal submucosa (SIS) has been shown to guide the specific regeneration of many tissues.
OBJECTIVE: To construct tissue-engineered skin by bone marrow mesenchymal stem cells (BMSCs) combined with SIS to treat full-thickness skin defects in rabbits with diabetes mellitus, and to observe the healing condition and effects.
METHODS: Full-thickness skin defect models were prepared in rabbits with diabetes mellitus. BMSCs with SIS were used to construct tissue-engineered skin for full-thickness skin defects in comparison with SIS scaffold and blank control group.
RESULTS AND CONCLUSION: The repairing area in the combination group was larger than that of SIS scaffold and blank control groups (P < 0.05). The rate of skin growth in the combination group was also faster than that of the other two groups. There were no skin appendage structures in the three groups. It was indicated that the satisfactory effect was obtained by tissue engineered skin transplantation, and the tissue engineered skin could be used to treat the full-thickness skin defects following diabetes mellitus.

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