Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 22-26.doi: 10.3969/j.issn.1673-8225.2012.01.004

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Effect of mesenchymal stem cells conditioned medium on protection of hydrogen peroxide injured cardiomyocytes in rats

Liu Xin-bin1, 2, Zhang Hong-chao2, Guo Zi-kuan3   

  1. 1Hebei North University, Zhangjiakou  075000, Hebei Province, China; 2Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing  100142, China; 3Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing  100850, China
  • Online:2012-01-01 Published:2012-01-01
  • Contact: Correspondence to: Zhang Hong-chao, Doctor, Associate chief physician, Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing 100142, Chinazhanghc2002@gmail.com
  • About author:Liu Xin-bin★, Studying for master’s degree, Physician, Hebei North University, Zhangjiakou 075000, Hebei Province, China; Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing 100142, China lxblp1237@126.com

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) transplantation can reduce myocardial infarct area and improve cardiac function. To date, most believe that MSCs play an important role in secreting biological factors.
OBJECTIVE: To observe the influence of MSCs conditioned medium (MSCs-CM) on cardiomyocytes apoptosis induced by hydrogen peroxide (H2O2).
METHODS: Rat MSCs and cardiomyocytes were isolated, cultured separately and identified. Preparation of MSCs-CM and H2O2 injured NRCs model. The rats were divided into four groups: control group, model group, MSCs-CM group and MSCs-CM+inhibitors of PI3K (Wortmannin) group. Lactate dehydrogenase (LDH) activity and apoptosis rates were detected by ELISA and flow cytometry separately in order to assess cardiomyocytes injury.
RESULTS AND CONCLUSIONS: LDH activity in the model group was significantly higher than that in the other groups (P < 0.001); While the LDH activity in the MSCs-CM+Wortmannin group was also higher than that in the MSCs-CM group (P < 0.001). The apoptosis rate in the MSCs-CM group was lower than that in the MSCs-CM+ Wortmannin group (P < 0.01), and the apoptosis rate in the two former groups was lower than that in the model group (P < 0.01). It indicated that MSC-CM inhibited H2O2-induced apoptosis of NRCs partially via PI3K pathway.

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