Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9885-9888.doi: 10.3969/j.issn.1673-8225.2011.53.002

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Proliferation and differentiation of recipient-sourced hepatic oval cells in liver graft after cell seeding

Li Zhu1, Chen Juan1, Li Xue-hua1, Liu Zhi-heng1, Liu Gui-jie1, Zhang Xue-li1, Gao Yan-chao1, Sun Hui-dong1,Ji Pi-you1, Li Li2, Zhang Jin-liang1   

  1. 1Department of Hepatopancreatobiliary Surgery, the People’s Hospital of Liaocheng, Liaocheng Hospital Affiliated to Taishan Medical University, Liaocheng  252000, Shandong Province, China
    2First Ward, Department of Hepatopancreatobiliary Surgery, the First People’s Hospital of Kunming, Ganmei Hospital Affiliated to Kunming Medical University, Kunming  650011, Yunnan Province, China
  • Received:2011-08-13 Revised:2011-09-05 Online:2011-12-31 Published:2011-12-31
  • Contact: Li Li, Professor, Doctoral supervisor, First Ward, Department of Hepatopancreatobiliary Surgery, the First People’s Hospital of Kunming, Ganmei Hospital Affiliated to Kunming Medical University, Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com.cn
  • About author:Li Zhu☆, Doctor, Attending physician, Department of Hepatopancreatobiliary Surgery, the People’s Hospital of Liaocheng, Liaocheng Hospital Affiliated to Taishan Medical University, Liaocheng 252000, Shandong Province, China lllllzhu@126.com

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Abstract:

BACKGROUND: Hepatic oval cells (HOCs) exhibit repair effects on liver injury after cell seeding.
OBJECTIVE: To observe the effects of recipient-sourced HOCs on survival time and liver function of recipient rats after liver transplantation and HOC seeding.
METHODS: Rats orthotopic liver transplantation models were established by Kamada two-cuff technique. Female DA rats served as donors, and female Lewis rats were used as recipients. Recipients were randomly divided into a control group (no cell seeding) and an experimental group (recipient-sourced HOCs seeding through portal vein and hepatic artery during liver transplantation, 1×109/L).
RESULTS AND CONCLUSION: The median survival time and accumulated survival rate in the experimental group were better than those in the control group (P < 0.05). Degree of hepatocyte injury in the experimental group was mild than that in the control group (P < 0.05), and liver synthetic function and liver excretion function in the experimental group were much better than those in the control group (P < 0.05). These findings suggest that seeding of recipient-sourced HOCs into liver graft can effectively improve liver function, obviously prolong median survival time and increase accumulated survival rate in recipients after liver transplantation. These results might be related to the proliferation and differentiation of recipient-sourced HOCs in liver graft, the repair of liver injury and alleviated acute rejection.

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