Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9561-9564.doi: 10.3969/j.issn.1673-8225.2011.51.015

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Repair of skin defect with collagen-chitosan compound nanofiber membrane

Yu Pei-jun1, 2, Wang Lu-ping3, Guo Yu3, Chen Wei3, Mo Xiu-mei4   

  1. 1College of Clinical Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
    2Department of Anesthesiology, the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    3the Eighth People’s Hospital of Shanghai, Shanghai  200235, China
    4College of Chemical Engineering and Biological Engineering, Donghua University, Shanghai  201620, China
  • Received:2011-07-14 Revised:2011-10-21 Online:2011-12-17 Published:2011-12-17
  • Contact: Chen Wei, Professor, Chief physician, the Eighth People’s Hospital of Shanghai, Shanghai 200235, China drchenwei@163.com Correspondence to: Mo Xiu-mei, Professor, College of Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China xmm@dhu.edu.cn
  • About author:Yu Pei-jun★, Master Physician, College of Clinical Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China; Department of Anesthesiology, the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China yupeijun82@163.com
  • Supported by:

    National High Technology Research and Development Program of China, No. 2008AA03Z305*

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Abstract:

BACKGROUND: Studies in vitro have confirmed that collagen-chitosan (80:20) compound nanofiber membranes have excellent mechanical properties, biocompatibility and biodegradability. However, there are few researches in vivo about the materials.
OBJECTIVE: To observe the effect of collagen-chitosan compound nanofiber membranes on repair of full-thickness skin defect on the back of Sprague Dawley (SD) rats.
METHODS: Thirty SD rats with full-thickness skin defects on the back were divided into two groups randomly by lottery method. SD rats treated with collogen-chitosan (80:20) compound nanofiber membrane and oily gauzes on the defects were taken as the experimental group, while those treated with oily gauzes only as the control group. The defects of all rats were fixed with dry gauze bandage at the margin of outer packaging.
RESULTS AND CONCLUSION: The wounds healed basically in the experimental group at 14 days after the repair; hematoxylin-eosin staining showed that the numbers of capillaries were reduced and the content of fibers was increased. While in the control group, wound healed irregularly, and the wound was larger than that in the experimental group; hematoxylin-eosin staining showed that there were more obvious dilated capillaries and a large number of inflammatory cells. The above results indicated that collagen-chitosan compound nanofiber membrane is superior to ordinary gauze in promoting the tissue repair and wound healing of skin defects.

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