Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9544-9548.doi: 10.3969/j.issn.1673-8225.2011.51.011

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Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissue-engineered blood vessels

Gao Guo-liang, Song Jian-fei, Wang Hai-yong, Zheng Min, Wang Wei, Jiang Yi-yao, Du Zhen-zong   

  1. Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-20 Revised:2011-06-29 Online:2011-12-17 Published:2011-12-17
  • Contact: Du Zhen-zong, Doctor, Associate professor, Associate chief physician, Master’s supervisor, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China duzhenzong@ sina.com
  • About author:Gao Guo-liang★, Studying for master’s degree, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China 26313597@qq.com
  • Supported by:

    the Healthy Department of Guangxi Zhuang Autonomous Region, No. Z2007199*; Z2008290*

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Abstract:

BACKGROUND: Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds.
OBJECTIVE: To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation.
METHODS: The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.
RESULTS AND CONCLUSION: Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.

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