Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7733-7736.doi: 10.3969/j.issn.2095-4344.2012.41.027

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Expression of totipotency associated genes cPouV, cNanog and Sox2 in early chicken embryo

Liu Ben-jie1, Chen Zhi-sheng2, Chen Sheng-feng2, Ji Hui-qin2, Wang Bing-yun2, Deng Xian-bai1   

  1. 1College of Veterinary Medicine of South China Agricultural University, Guangzhou 510642, Guangdong Province, China
    2School of Life Science, Foshan University, Foshan 528231, Guangdong Province, China
  • Received:2012-01-24 Revised:2012-02-23 Online:2012-10-07 Published:2013-04-09
  • Contact: Deng Xian-bai, Associate professor, College of Veterinary Medicine of South China Agricultural University, Guangzhou 510642, Guangdong Province, China bywang63@163.com Wang Bin-yun, Doctor, Professor, School of Life Science, Foshan University, Foshan 528231, Guangdong Province, China bywang63@163.com
  • About author:Liu Ben-jie★, Studying for master’s degree, College of Veterinary Medicine of South China Agricultural University, Guangzhou 510642, Guangdong Province, China 06160810@163.com

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Abstract:

BACKGROUND: Studies have confirmed that totipotency associated genes cPouV, cNanog and Sox2 play an important role in regulating the pluripotency and self-renewal of chicken embryonic stem cells, but there lack studies on the expression and effect of the genes in early chicken embryo.
OBJECTIVE: To detect the expression levels of cPouV, cNanog and Sox2 in early chicken embryo, and to find the effect of the genes in early chicken embryonic development.
METHODS: 180 fresh fertilized eggs were divided into six groups, and took out after incubated for 0, 8, 16, 22, 32 and 42 hours. The embryo samples were obtained, and the real-time quantitative PCR was used to detect the expression levels of cPouV, cNanog and Sox2 with the internal control gene of β-actin.
RESULTS AND CONCLUSION: The relative expressions of cNanog and cPouV have the same changes, the relative expressions of cNanog and cPouV were increased after incubated for 0, 8, 16 and 22 hours, and reached to peak after incubated for 22 hours and then decreased gradually. The relative expression of Sox2 showed a significant increasing trend. The totipotency associated genes of cPouV, cNanog and Sox2 have an important role in regulating the early embryonic development, but the effects are not exactly the same.

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