Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9223-9226.doi: 10.3969/j.issn.1673-8225.2011.49.024

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Differentiation of placenta mesenchymal stem cells to epidermal cells in vitro

Wan Zhen-zhou1, Feng Ya-song1, Peng Hai-lin2, Zhang Wei1, Miao Zong-ning3, Chen Ya-bao2   

  1. 1Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou  225300, Jiangsu Province, China
    2Medical Laboratory of Taizhou People's Hospital, Taizhou  225300, Jiangsu Province, China
    3Stem Cell Laboratory of Wuxi Third People's Hospital, Wuxi  214000, Jiangsu Province, China
  • Received:2011-06-26 Revised:2011-11-10 Online:2011-12-03 Published:2011-12-03
  • About author:Wan Zhen-zhou, Docimaster in charge, Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou 225300, Jiangsu Province, China 13901433616@139.com

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Abstract:

BACKGROUND: The pervious study has showed that placenta mesenchymal stem cells (PMSCs) can differentiate into bone, cartilage, and muscle and adipose mesenchymal stem cells.
OBJECTIVE: To investigate the differentiation of PMSCs into epidermal cells in vitro.
METHODS: PMSCs were isolated and cultured and the surface antigen was detected by flow cytometry.
RESULTS AND CONCLUSION: Isolated PMSCs were positive for the markers CD105, CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34, CD45, CD106 and HLA-DR. The morphology of induced cells was obviously changed. Immunohistochemistry stain analysis showed positive for epidermal-specific markers like CK19 and CK10. The PMSCs have the capability to differentiate into epidermal cells in vitro. The placenta may prove to be a useful source of mesenchymal stem cells in skin tissue engineering and stem cells study.

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