Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9174-9177.doi: 10.3969/j.issn.1673-8225.2011.49.012

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Differentiation of rat embryonic cerebral cortex neural stem cells cultured by erythropoietin in vitro

Yuan Li-li1, Du Hong-mei2, Guan Ying-jun2, Kong You-hua1   

  1. 1Department of Histology and Embryology of Jining Medical College, Jining  272067, Shandong Province, China
    2Department of Histology and Embryology of Weifang Medical College, Weifang  261053, Shandong Province, China
  • Received:2011-06-18 Revised:2011-10-20 Online:2011-12-03 Published:2011-12-03
  • About author:Yuan Li-li★, Master, Lecturer, Department of Histology and Embryology of Jining Medical College, Jining 272067, Shandong Province, China liliyuan79@yahoo.com.cn
  • Supported by:

    Youth Research Foundation of Jining Medical University, No. 2008jnqn-25*

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Abstract:

BACKGROUND: Erythropoietin (FPO) is early known as a kind of growth factor. In recent years, there are many studies about the protective effect of erythropoietin on the central nervous system in vivo.
OBJECTIVE: To explore the effects of EPO on the apoptosis and differentiation of rat embryonic cerebral cortex neural stem cells in vitro.
METHODS: The embryonic cerebral cortex were isolate from Sprague-Dawley rats that pregnant for 14 days under sterile conditions, the cells were cultured and differentiated in the suspension medium and then adherent induction. The neural stem cells were detected by nestin immune cell fluorescence staining, microtubule-associated protein 2 (MAP-2) and glia fibrillary acid protein was used to detect the differentiation of neural stem cells. The third passage of neural stem cells were obtained and 0.5, 5, 50, 500 U/mL EPO were added into the medium, and in the control group, there was no EPO adding. Caspase-3 was used to detect the apoptosis of neural stem cells, and MAP-2 was used to detect the differentiation of neural stem cells to neuron.
RESULTS AND CONCLUSION: The expression of the caspase-3 in neural sphere was decreased after added EPO (≥5 U/mL), and the expression of the MAP-2 positive cells was increased obviously after differentiation (P < 0.01). The present results suggest that EPO can decrease the apoptosis of the neural stem cells and promote neural stem cells to differentiate into neurons in vitro.

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