Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9141-9145.doi: 10.3969/j.issn.1673-8225.2011.49.005

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Bone marrow mesenchymal stem cells modified by glial cell line-derived neural factor gene co-cultrued with neurons subjected to anoxia-reoxygenation

Xiong Huai-lin1, Deng Li 2, Gao Xiao-qing2, Wang Te-wei2, Guo Kan2, Yang Chao-xian 2   

  1. 1Department of Anatomy, 2Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2011-06-16 Revised:2011-08-10 Online:2011-12-03 Published:2011-12-03
  • Contact: Yang Chao-xian, Professor, Master’s supervisor, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China yangchaoxian2006@tom.com
  • About author:Xiong Huai-lin☆, Studying for doctorate, Lecturer, Department of Anatomy, Luzhou Medical College, Luzhou 646000, Sichuan Province, China 497101029@qq.com
  • Supported by:

     the Foundation of Sichuan Science and Technology Department, No.2006J13-005-1*;  the Foundation of Sichuan Educational Department, No. 2006A155*

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Abstract:

BACKGROUND: Preliminary studies in workgroup indicated that glial-derived neurotrophic factor (GDNF) gene modified by bone marrow mesenchymal stem cells (BMSCs) (BMSCs/GDNF) can stably express the mRNA of GDNF, but whether GDNF protein in BMSCs/GDNF affects neurons subjected to anoxia-reoxygenation positively is unclear.
OBJECTIVE: To observe the protective effects of BMSCs/GDNF on neurons subjected to anoxia-reoxygenation.
METHODS: BMSCs/GDNF were co-cultured with anoxia-reoxygenation nerve cells. Hoechst33258 staining was used to detect apoptosis of neurons. Immunofluorescent-chemistry method was used to detect growth-associated protein-43 (GAP-43) expression in co-cultured cells.
RESULTS AND CONCLUSION: The rate of neurons apoptosis in the BMSCs/GDNF group was lower than that of the BMSCs group and anoxia group (P < 0.05). Absorbance of growth-associated protein-43 (GAP-43) expression in the BMSCs/GDNF group was higher than that in the BMSCs group (P < 0.05). It is indicated that BMSCs/GDNF have neuroprotective effects due to inhibiting apoptosis of neurons subjected to anoxia-reoxygenation and increasing GAP-43 expression in co-cultured cells.

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