Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8595-8597.doi: 10.3969/j.issn.1673-8225.2011.46.011

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Effects of Jinwugutong capsule on the expression of interleukin1, 6 in osteoblasts

Liu Chun-ying1, Zheng Wen-kui2, Zu Jin-chi2, Zhou Yu-juan1, Liu Li1, Wang En-jun1, Li Shao-chun1   

  1. 1Department of Pharmacology, Hebei University Health Science Center, Baoding 071000, Hebei Province, China
    2 Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • Received:2011-06-25 Revised:2011-07-04 Online:2011-11-12 Published:2011-11-12
  • About author:Liu Chun-ying★, Master, Lecturer, Department of Pharmacology, Hebei University Health Science Center, Baoding 071000, Hebei Province, China liuchy310@163.com
  • Supported by:

    Scientific Research Development Project of Administration of Traditional Chinese Medicine of Hebei Province, No. 2009149*

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Abstract:

BACKGROUND: Our previous studies have shown that Jinwugutong capsule exhibits obvious effects on postmenopausal osteoporosis.
OBJECTIVE: To investigate the effects of Jinwugutong capsule-containing serum on the expression of interleukin (IL)-1 and IL-6 in osteoblasts.
METHODS: Bilateral ovaries of female Sprague-Dawley (SD) rats were removed to prepare ovarian serum. Ovariotomized (OVX) rats were intragastrically administered Jinwugutong capsule to prepare ovarian medicated serum. Passage 3 osteoblasts which were harvested from rats born within 24 hours were cultured with normal female rat serum (normal serum group), OVX rat serum (OVX serum group) and OVX rat Jinwugutong capsule-containing serum (OVX drug-containing serum group) for 72 hours separately.  
RESULTS AND CONCLUSION: ELISA results showed that IL-1 and IL-6 expression in the osteoblasts was significantly increased in the OVX serum group than in the normal serum group (P < 0.01); IL-1 and IL-6 expression in the osteoblasts was significantly decreased in the OVX drug-containing serum group than in the OVX serum group, but there was no significant difference in IL-1 and IL-6 expression between normal serum group and OVX drug-containing serum group. These findings suggest that Jinwugutong capsule can prevent and treat osteoporosis by inhibiting IL-1 and IL-6 expression in osteoblasts. 

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