Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8591-8594.doi: 10.3969/j.issn.1673-8225.2011.46.010

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Effects of hypoxia on proliferation and differentiation of rat osteoblasts

Gao Wen-kui, Wang De-yuan, Li Zhi-gang, Yan Zi-qiang, Bai Feng, Wang Wei   

  1. Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China
  • Received:2011-07-28 Revised:2011-08-26 Online:2011-11-12 Published:2011-11-12
  • Contact: Wang Wei, Master, Attending physician, Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China warerry@163.com
  • About author:Gao Wen-kui★, Master, Chief physician, Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China osteo4pla@163.com
  • Supported by:

    Science and Technology Innovation Ability Promotion Plan Program of Department of Science and Technology of Qinghai Province, No. 2010.Z.744*

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Abstract:

BACKGROUND: Hypoxia influences bone metabolism by many means and exhibits negative effects on bone generation and bone healing.
OBJECTIVE: To explore the effects of hypoxia on proliferation and differentiation of rat osteoblasts and investigate the underlying mechanisms.
METHODS: Primary rat osteoblasts were isolated from excised calvarial bones of neonatal mice and cultured in vitro. The second passage of osteoblasts were cultured under hypoxic (3% O2) and normoxic (20% O2) conditions.
RESULTS AND CONCLUSION: The proliferation levels, alkaline phosphatase activities, osteocalcin contents and the number of calcium nodules in the hypoxic group were significantly lower compared with the normoxic group (P < 0.05 or P < 0.01). It means that hypoxia inhibits the proliferation, differentiation and functions of rat osteoblasts. The mRNA expression of bone morphogenetic protein-2 and Runx2 in osteoblasts cultured in 3% O2 was lower than that cultured in 20% O2 ( P < 0.05 or      P < 0.01). It means that the mRNA expression of Runx2 and bone morphogenetic protein-2 was decreased in hypoxic conditions. These findings suggest that hypoxia can inhibit the proliferation and differentiation of rat osteoblasts through inhibiting Runx2 and BMP-2 mRNA expression in vitro.

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