Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (45): 8371-8375.doi: 10.3969/j.issn.1673-8225.2011.45.003

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Effect of bone marrow mesenchymal stem cells on down-regulation of Toll-like receptor 8 and Toll-like receptor 9 in spleen mononuclear cells in collagen-induced arthritis mice

Mao Fei, Qian Hui, Zhu Wei, Yan Yong-min, Gao Shuo, Xu Wen-rong   

  1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
  • Received:2011-04-08 Revised:2011-06-21 Online:2011-11-05 Published:2011-11-05
  • Contact: Xu Wen-rong, Doctor, Professor, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China icls@ujs.edu.cn
  • About author:Mao Fei☆, Doctor, Lecturer, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China maofei2003@ujs.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 81000181*; the Natural Science Foundation of Jiangsu Universities, No. 10KJB310002*; the Science and Technology Start-up Foundation for Advance Talents of Jiangsu University, No. 10JDG094*; Top-Notch Talent and Technological Innovation Team Project of Jiangsu University, No. 2008-018-02*

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) can attenuate the symptoms of collagen-induced arthritis (CIA) mice but the mechanism of which is not clear.
OBJECTIVE: To explore the effect of MSCs on the expression of Toll-like receptor 8 (TLR8), Toll-like receptor 9 (TLR9) and so on in spleen mononuclear cells in CIA mice.
METHODS: DBA/1J mice were randomly divided into sham-operation group, positive control group and test group. Chicken Ⅱ collagen was used to establish the model of CIA in mice. Rat MSCs were administered into the test group through intravenous injection.
RESULTS AND CONCLUSION: The joint diameter of the test mice decreased obviously compared with that of positive control group. Severe inflammation was detected in the positive group mice by histological analysis, which was not seen in the test group and negative group. The expression levels of TLR8, TLR9 and interleukin-1β in spleen mononuclear cells from the test group were decreased compared with those from the positive group which were higher than that from the negative group (P < 0.01, P < 0.05). Statistical analysis showed that there was no correlation between the expression of TLR8 and TLR9 in the positive control group and test group (P > 0.05). The expression of TLR8 and TLR9 in spleen mononuclear cells from CIA mice were decreased by MSCs, and there was no correlation between the expression of TLR8 and TLR9.

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