Abstract:

BACKGROUND: Exercise-induced fatigue is a hot topic in the field of sports medicine, and whether there is some association between exercise-induced fatigue occurrence and recovery and cerebrospinal fluid circulation remains poorly understood.
OBJECTIVE: To study the dynamic changes and correlation of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in cerebral cortex and cerebrospinal fluid of rats after movement with increasing loads.
METHODS: 30 male SD rats were evenly divided into control group (C), immediately after incremental exercise group (E1), 24 hours after incremental exercise (E2). The control group rats were conventionally fed, and the E1 and E2 group rats received 6 weeks of incremental exercise and then sacrificed immediately and 24 hours after exercise. Cerebrospinal fluid was extracted and cerebral cortex was taken. NE and 5-HT levels in cerebral cortex and cerebrospinal fluid were detected by high-performance liquid chromatography-fluorescence detection.
RESULTS AND CONCLUSION: The NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid in the E1 group were significantly higher compared with the C group (P < 0.01). The NE and 5-HT levels in the cerebral cortex were significantly higher in the E2 group compared with the C group (P < 0.01), but there were no significant differences in NE and 5-HT levels in cerebrospinal fluid between the E2 group and the C group. In the E1 group, NE and 5-HT levels in the cerebral cortex were significantly positively correlated (P < 0.05) and NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid were highly correlated (P < 0.01). Results showed that immediately and 24 hours after incremental exercise, the excited effect of NE and the inhibitory effect of 5-HT were in a dynamic balance and that immediately after incremental exercise, the dynamic changes of NE and 5-HT in the cerebrospinal fluid can reflect the dynamic changes of NE and 5-HT in the cerebral cortex.